Human pan-genome result

Done Task link: 8fe919c6-6147-4841-b32e-0c83bebc9c01

Primer pair

Primer ID Sequence (5' → 3') Size (bp) GC (%) Tm (°C) ΔG (kcal/mol)
HLA-DRB-AMP-A CCCCACAGCACGTTTCTTG 19 57.89 59.84 -21.50
HLA-DRB-AMP-B CCGCTGCACTGTGAAGCTCT 20 60.00 63.57 -23.79

Coverage across pan-genome

Human pan-genome (97 assemblies, HPRC / GCA softmasked)

100.0%
Coverage
97 / 97
97
With amplicon (≥1)
0
No amplicon
Multi-amp: 84

download summary.tsv

Per-assembly table

Assembly Status #Amp Size(s) Locus Note
GCA_009914755.4 Multi 2 274,274 6:32350242-32350516; 6:32413950-32414224
GCA_018466835.1 Multi 2 274,274 JAGYVI010000022.1:27884384-27884658; JAGYVI010000022.1:27948082-27948356
GCA_018466845.1 Multi 2 274,274 JAGYVH010000080.1:5816965-5817239; JAGYVH010000080.1:5791128-5791402
GCA_018466855.1 Multi 2 274,274 JAGYVK010000047.1:32542290-32542564; JAGYVK010000047.1:32671347-32671621
GCA_018466985.1 Multi 2 274,274 JAGYVJ010000064.1:32540274-32540548; JAGYVJ010000064.1:32476637-32476911
GCA_018467005.1 Multi 2 274,275 JAGYVM010000005.1:26870213-26870487; JAGYVM010000005.1:26834198-26834473
GCA_018467015.1 Multi 2 274,274 JAGYVL010000026.1:32511942-32512216; JAGYVL010000026.1:32448009-32448283
GCA_018467155.1 Multi 2 274,274 JAGYVN010000027.1:27516776-27517050; JAGYVN010000027.1:27542590-27542864
GCA_018467165.1 Multi 2 274,275 JAGYVO010000024.1:26428830-26429104; JAGYVO010000024.1:26392815-26393090
GCA_018469405.1 Multi 2 274,274 JAGYYU010000011.1:26636071-26636345; JAGYYU010000011.1:26665640-26665914
GCA_018469415.1 Multi 2 274,274 JAGYYT010000073.1:23589023-23589297; JAGYYT010000073.1:23525078-23525352
GCA_018469425.1 Multi 2 274,274 JAGYYS010000003.1:32690480-32690754; JAGYYS010000003.1:32626901-32627175
GCA_018469665.1 Multi 2 274,274 JAGYYY010000050.1:32564415-32564689; JAGYYY010000050.1:32500838-32501112
GCA_018469675.1 Multi 2 274,274 JAGYYV010000066.1:27573011-27573285; JAGYYV010000066.1:27636674-27636948
GCA_018469685.1 OK 1 274 JAGYYW010000059.1:32545341-32545615
GCA_018469695.1 OK 1 274 JAGYYZ010000057.1:26437938-26438212
GCA_018469705.1 Multi 2 274,274 JAGYYX010000108.1:7342797-7343071; JAGYYX010000108.1:7406384-7406658
GCA_018469865.1 Multi 2 274,274 JAGYZA010000082.1:7356883-7357157; JAGYZA010000082.1:7420650-7420924
GCA_018469875.1 Multi 2 274,274 JAHAON010000041.1:32525384-32525658; JAHAON010000041.1:32654310-32654584
GCA_018469925.1 OK 1 274 JAHAOO010000042.1:28037948-28038222
GCA_018469935.1 Multi 2 274,274 JAHAOR010000061.1:23675177-23675451; JAHAOR010000061.1:23546255-23546529
GCA_018469945.1 Multi 2 274,274 JAHAOQ010000088.1:16412278-16412552; JAHAOQ010000088.1:16283308-16283582
GCA_018469955.1 Multi 2 274,274 JAHAOP010000058.1:23662127-23662401; JAHAOP010000058.1:23533151-23533425
GCA_018469965.1 Multi 2 274,274 JAGYZB010000008.1:7473838-7474112; JAGYZB010000008.1:7344767-7345041
GCA_018470425.1 Multi 2 274,274 JAHAOS010000073.1:5832985-5833259; JAHAOS010000073.1:5769075-5769349
GCA_018470435.1 Multi 2 274,274 JAHAOW010000052.1:584006-584280; JAHAOW010000052.1:647594-647868
GCA_018470445.1 Multi 2 274,274 JAHAOV010000201.1:5664877-5665151; JAHAOV010000201.1:5728668-5728942
GCA_018470455.1 Multi 2 274,274 JAHAOT010000003.1:26147804-26148078; JAHAOT010000003.1:26018820-26019094
GCA_018470465.1 Multi 2 274,274 JAHAOU010000006.1:22918001-22918275; JAHAOU010000006.1:22981935-22982209
GCA_018471065.1 Multi 2 274,275 JAHAMA010000069.1:7415573-7415847; JAHAMA010000069.1:7379560-7379835
GCA_018471075.1 Multi 2 274,274 JAHAMC010000024.1:7340123-7340397; JAHAMC010000024.1:7403721-7403995
GCA_018471085.1 Multi 2 274,274 JAHALZ010000032.1:23757260-23757534; JAHALZ010000032.1:23783095-23783369
GCA_018471095.1 Multi 2 274,274 JAHALX010000077.1:26046962-26047236; JAHALX010000077.1:26110764-26111038
GCA_018471105.1 Multi 2 274,274 JAHALY010000025.1:23763171-23763445; JAHALY010000025.1:23826870-23827144
GCA_018471345.1 Multi 2 274,274 JAHAMB010000019.1:116410400-116410674; JAHAMB010000019.1:116473993-116474267
GCA_018471515.1 Multi 2 274,274 JAHBCA010000042.1:23896963-23897237; JAHBCA010000042.1:23767820-23768094
GCA_018471525.1 Multi 2 274,274 JAHAMG010000076.1:23537866-23538140; JAHAMG010000076.1:23601771-23602045
GCA_018471535.1 Multi 2 274,274 JAHAMF010000034.1:3750560-3750834; JAHAMF010000034.1:3814158-3814432
GCA_018471545.1 Multi 2 274,274 JAHAMD010000016.1:32536314-32536588; JAHAMD010000016.1:32665378-32665652
GCA_018471555.1 Multi 2 274,274 JAHAME010000044.1:27853794-27854068; JAHAME010000044.1:27917703-27917977
GCA_018472565.1 OK 1 274 JAHBBY010000031.1:1067118-1067392
GCA_018472575.1 Multi 2 274,274 JAHBCD010000020.1:23888833-23889107; JAHBCD010000020.1:23760915-23761189
GCA_018472585.1 Multi 2 274,274 JAHBBZ010000030.1:32360914-32361188; JAHBBZ010000030.1:32424619-32424893
GCA_018472595.1 Multi 2 274,274 JAHBCB010000040.1:23763895-23764169; JAHBCB010000040.1:23827433-23827707
GCA_018472605.1 Multi 2 274,274 JAHBCC010000005.1:32447541-32447815; JAHBCC010000005.1:32511235-32511509
GCA_018472685.1 Multi 2 274,274 JAHBCE010000076.1:7288936-7289210; JAHBCE010000076.1:7352526-7352800
GCA_018472695.1 Multi 2 274,274 JAGYVP010000017.1:32475706-32475980; JAGYVP010000017.1:32603656-32603930
GCA_018472705.1 Multi 2 274,274 JAGYVQ010000020.1:27909121-27909395; JAGYVQ010000020.1:27780064-27780338
GCA_018472715.1 Multi 2 274,274 JAHBCH010000013.1:32637310-32637584; JAHBCH010000013.1:32611483-32611757
GCA_018472725.1 OK 1 274 JAHBCF010000017.1:221075-221349
GCA_018472765.1 Multi 2 274,274 JAHBCG010000038.1:3878082-3878356; JAHBCG010000038.1:4007139-4007413
GCA_018472825.1 Multi 2 274,274 JAGYVT010000002.1:3891711-3891985; JAGYVT010000002.1:3827778-3828052
GCA_018472835.1 Multi 2 274,274 JAGYVU010000035.1:17335131-17335405; JAGYVU010000035.1:17399064-17399338
GCA_018472845.1 OK 1 275 JAGYVS010000035.1:32552989-32553264
GCA_018472855.1 Multi 2 274,274 JAGYVV010000230.1:3735221-3735495; JAGYVV010000230.1:3798817-3799091
GCA_018472865.1 Multi 2 274,274 JAGYVR010000046.1:1986283-1986557; JAGYVR010000046.1:1858392-1858666
GCA_018473295.1 OK 1 274 JAGYVX010000013.1:23771461-23771735
GCA_018473305.1 Multi 2 274,274 JAGYVW010000148.1:1890662-1890936; JAGYVW010000148.1:1761686-1761960
GCA_018473315.1 Multi 2 274,274 JAGYVY010000082.1:17348755-17349029; JAGYVY010000082.1:17378326-17378600
GCA_018503245.1 Multi 2 274,274 JAHEOQ010000034.1:23750614-23750888; JAHEOQ010000034.1:23814547-23814821
GCA_018503255.1 Multi 2 274,274 JAHEON010000020.1:23744966-23745240; JAHEON010000020.1:23808545-23808819
GCA_018503265.1 Multi 2 274,274 JAHEOM010000153.1:1845786-1846060; JAHEOM010000153.1:1909390-1909664
GCA_018503275.1 OK 1 275 JAHEOL010000047.1:3940820-3941095
GCA_018503285.1 Multi 2 274,274 JAHEOO010000017.1:28847345-28847619; JAHEOO010000017.1:28783405-28783679
GCA_018503525.1 Multi 2 274,274 JAHEOP010000002.1:3765273-3765547; JAHEOP010000002.1:3829044-3829318
GCA_018503575.1 Multi 2 274,274 JAHEOS010000026.1:3920283-3920557; JAHEOS010000026.1:3856703-3856977
GCA_018503585.1 Multi 2 274,274 JAHEOR010000019.1:17381563-17381837; JAHEOR010000019.1:17317617-17317891
GCA_018504045.1 Multi 2 274,274 JAHEOY010000117.1:3770894-3771168; JAHEOY010000117.1:3834667-3834941
GCA_018504055.1 Multi 2 274,274 JAHEOW010000032.1:32536020-32536294; JAHEOW010000032.1:32599612-32599886
GCA_018504065.1 OK 1 274 JAHEOT010000107.1:23677465-23677739
GCA_018504075.1 Multi 2 274,274 JAHEOU010000100.1:5384109-5384383; JAHEOU010000100.1:5447698-5447972
GCA_018504085.1 OK 1 274 JAHEOV010000002.1:23904942-23905216
GCA_018504365.1 Multi 2 274,274 JAHEOZ010000001.1:27956732-27957006; JAHEOZ010000001.1:27982546-27982820
GCA_018504375.1 Multi 2 274,274 JAHEOX010000097.1:27030024-27030298; JAHEOX010000097.1:27093601-27093875
GCA_018504625.1 Multi 2 274,274 JAHEPE010000077.1:3762756-3763030; JAHEPE010000077.1:3826691-3826965
GCA_018504635.1 Multi 2 274,274 JAHEPD010000054.1:23528864-23529138; JAHEPD010000054.1:23592766-23593040
GCA_018504645.1 OK 1 274 JAHEPA010000084.1:27453948-27454222
GCA_018504655.1 Multi 2 274,274 JAHEPB010000021.1:3672523-3672797; JAHEPB010000021.1:3736297-3736571
GCA_018504665.1 Multi 2 274,275 JAHEPC010000026.1:3793874-3794148; JAHEPC010000026.1:3829886-3830161
GCA_018505825.1 OK 1 274 JAHEPF010000055.1:1837541-1837815
GCA_018505835.1 Multi 2 274,275 JAHEPI010000049.1:16331726-16332000; JAHEPI010000049.1:16295713-16295988
GCA_018505845.1 Multi 2 274,274 JAHEPH010000100.1:3766408-3766682; JAHEPH010000100.1:3830000-3830274
GCA_018505855.1 OK 1 274 JAHEPK010000074.1:3824849-3825123
GCA_018505865.1 Multi 2 274,274 JAHEPG010000124.1:3805690-3805964; JAHEPG010000124.1:3741782-3742056
GCA_018506125.1 Multi 2 274,274 JAHEPJ010000058.1:23518406-23518680; JAHEPJ010000058.1:23581997-23582271
GCA_018506155.1 Multi 2 274,274 JAHEPM010000086.1:23063726-23064000; JAHEPM010000086.1:22934738-22935012
GCA_018506165.1 Multi 2 274,274 JAHEPL010000070.1:23039127-23039401; JAHEPL010000070.1:22910039-22910313
GCA_018506945.1 Multi 2 274,274 JAHEPO010000054.1:5695846-5696120; JAHEPO010000054.1:5824990-5825264
GCA_018506955.1 Multi 2 274,274 JAHEPQ010000070.1:32468479-32468753; JAHEPQ010000070.1:32532178-32532452
GCA_018506965.1 Multi 2 274,274 JAHEPN010000064.1:7416664-7416938; JAHEPN010000064.1:7544575-7544849
GCA_018506975.1 Multi 2 274,274 JAHEPP010000060.1:27291925-27292199; JAHEPP010000060.1:27355522-27355796
GCA_018852585.1 Multi 2 274,274 JAHKSF010000005.1:3919828-3920102; JAHKSF010000005.1:3856249-3856523
GCA_018852595.1 Multi 2 274,275 JAHKSG010000017.1:7414647-7414921; JAHKSG010000017.1:7378631-7378906
GCA_018852605.1 Multi 2 274,274 JAHKSE010000066.1:5819607-5819881; JAHKSE010000066.1:5948731-5949005
GCA_018852615.1 Multi 2 274,274 JAHKSD010000045.1:26577614-26577888; JAHKSD010000045.1:26607183-26607457
GCA_021950905.1 Multi 2 274,274 6:32488260-32488534; 6:32617384-32617658
GCA_021951015.1 Multi 2 274,274 6:32508310-32508584; 6:32478741-32479015

Parameters

Tm min (°C)40
Size (bp)0 – 500
Mono / Diva / dNTP / Oligo50 / 1.5 / 0.25 / 50
🧬 Powered by
iGeneTech Bioscience

Target Gene Enrichment Expert

Custom NGS Panels Library Prep Kits
Explore Products →
Contact
Request more input?

For bug reports or suggestions, please email us.