Specificity analysis

Number of primer sequences should <= 50, degenerate primers should <= 2. Otherwise, please use the local command-line version.
Multiple selection supported (<= 5).
Min
°C
Minimum Tm value required for the binding stability between primer and its binding sites.
From
To
Product size: 0 (min) - 100000 (max).

Accurate but slow, new in version 3.0.
mM
mM
mM
nM