Human pan-genome primer check

Introduction Example result

Check whether one primer pair can amplify across the human pan-genome set (Human pan-genome (97 assemblies, HPRC / GCA softmasked)). The database is fixed; all indexed assemblies are analyzed.

Exactly one forward and one reverse primer. For more primers, use Specificity analysis or the local command-line version.
Default pair (HLA-DRB-AMP-A / AMP-B, ~274 bp) are the 11th IHW standard DRBAMP-A / DRBAMP-B primers (Table 1 in Bhattacharya et al., 2013; PMID 23441825).
Human pan-genome (97 assemblies, HPRC / GCA softmasked)
Source: Human Pangenome Reference Consortium (HPRC) @ Ensembl — download links for the assemblies used here.
No database selection — every indexed assembly in this set is checked.

Potential amplicon settings

Min
°C
Minimum Tm for primer–template binding.
From
To
Product size window.

Thermodynamic settings

mM
mM
mM
nM

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