MFEprimer-4.2.0: PCR Primer Design and Quality Control Tools

* Primer sequences (<= 2) (FASTA format or just plain sequences one per line, example, clear) Number of primer sequences should <= 2. Otherwise, please use the local command-line version.

Database Species.

* Background Database Multiple selection supported (<= 5).

Selected Background Databases

Potential amplicon settings

T_m

Min

°C

Minimum T_m value required for the binding stability between primer and its binding sites.

Size (bp)

From

Product size: 0 (min) - 100000 (max).

Allow mismatch at the 3' end

Thermodynamic settings

Concentration of monovalent cations (usually KCl)

Concentration of divalent cations (usually MgCl₂)

Concentration of dNTPs

Annealing oligo concentration

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Coverage analysis for various microbial strains