MFEprimer-4.2.0: PCR Primer Design and Quality Control Tools

Genome database

Targets in BED format (<= 100, example, clear) Maximum 100 lines, each region ≤ 10M bp

Chromosome name examples

chrM    1000 1001
chrM    1000 2000
chrM    3000 3100

Line width 0 to keep one line per sequence

Left flank (bp) Add to smaller position

Right flank (bp) Add to larger position

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Retrieve subsequence from FASTA