* Primer sequences (<= 1000?) (FASTA format or just plain sequences one per line, example, clear)

Potential dimer settings

Allowed max mismatches for a dimer

Max

Dimer type

All types (strict)

3' end binding only

Score (base pairs)

Min

Score (alignment base pairs) cutoff value to filter dimers

ΔG

Max

kcal/mol

ΔG cutoff value to filter dimers

Thermodynamic settings

Concentration of monovalent cations (usually KCl)

mM

Concentration of divalent cations (usually MgCl₂)

mM

Concentration of dNTPs

mM

Annealing oligo concentration

nM

Run