MFEprimer-4.4.0: PCR Primer Design and Quality Control Tools

* Primer sequences (<= 1000?) (FASTA format or just plain sequences one per line, example, clear)

Potential dimer settings

Allowed max mismatches for a dimer

Max

Dimer type

All types (strict)

3' end binding only

Score (base pairs)

Min

Score (alignment base pairs) cutoff value to filter dimers

ΔG

Max

kcal/mol

ΔG cutoff value to filter dimers

Thermodynamic settings

Concentration of monovalent cations (usually KCl)

Concentration of divalent cations (usually MgCl₂)

Concentration of dNTPs

Annealing oligo concentration

🧬 Powered by

Target Gene Enrichment Expert

Contact

Registration is optional. All tools work without an account. Login or register only if you want to manage long-running jobs under your account.

For bug reports or suggestions, please email us.

We use cookies to personalize your experience.

All services provided on our server are available free of charge for both individuals and businesses.

All task data will be deleted after 180 days.

Dimer analysis