total size: 6 bp, cov size: 5, cov rate: 83.33%, miss size: 1, miss rate: 16.67%, download coverage report.
| Chrom | Start | End | Size (bp) | CovSize (bp) | CovRate (%) | MissSize (bp) | MissRate (%) | |
|---|---|---|---|---|---|---|---|---|
| 1 | chr1 | 43554152 | 43554153 | 1 | 1 | 100.00 | 0 | 0.00 |
| 2 | chr1 | 45502740 | 45502741 | 1 | 0 | 0.00 | 1 | 100.00 |
| 3 | chr1 | 55148469 | 55148470 | 1 | 1 | 100.00 | 0 | 0.00 |
| 4 | chr1 | 55914417 | 55914418 | 1 | 1 | 100.00 | 0 | 0.00 |
| 5 | chr1 | 55987778 | 55987779 | 1 | 1 | 100.00 | 0 | 0.00 |
| 6 | chr1 | 55987806 | 55987807 | 1 | 1 | 100.00 | 0 | 0.00 |
tube: 1, primers: 4, product size range (bp): 283 - 300, download primers.
| ID | TemplateID | FpTm (°G) | RpTm (°G) | FpSize (bp) | RpSize (bp) | AmpSize (bp) | AmpGC (%) |
|---|---|---|---|---|---|---|---|
| T1P1 | chr1:43554152-43554153 | 59.45 | 59.22 | 22 | 19 | 300 | 60.00 |
| T1P2 | chr1:55148469-55148470 | 59.39 | 59.64 | 23 | 23 | 287 | 27.87 |
| T1P3 | chr1:55914417-55914418 | 59.88 | 59.88 | 27 | 27 | 300 | 41.33 |
| T1P4 | chr1:55987778-55987779 | 60.76 | 60.17 | 22 | 21 | 283 | 41.70 |
| Target Database | mm10.fa |
| Validation method | ngs |
| Product Size Min Difference | 0 |
| Primer Min Size (bp) | 16 |
| Primer Opt Size (bp) | 22 |
| Primer Max Size (bp) | 32 |
| Primer Min GC (%) | 20 |
| Primer Opt GC (%) | 50 |
| Primer Max GC (%) | 82 |
| Primer Min Tm (°C) | 58 |
| Primer Opt Tm (°C) | 60 |
| Primer Max Tm (°C) | 62 |
| Product Min Size (bp) | 160 |
| Product Max Size (bp) | 300 |
| Product Min GC (%) | 25 |
| Product Opt GC (%) | 50 |
| Product Max GC (%) | 78 |
| Dimer ΔG (kcal/mol) | -3.50 |
| Hairpin Score | 5 |
| Non-specific Amplicon Tm (°C) | 57 |
| Non-specific Amplicon Min Size (bp) | 0 |
| Non-specific Amplicon Max Size (bp) | 350 |
| Min Off-targets | 0 |
| Max Off-targets | 10 |
| Minimum Diffierent Bases | 50 |
| SNP Skip Size | 9 |
| InDel Skip Size | 15 |
| Background Database 1 | hg38.fa |
| Background Database 2 | - |
| Min Amplicon Count on Background Genomes | 1 |
| Max Amplicon Count on Background Genomes | 1 |