Primer Design Reports (2025-07-21 17:52:24)
[0-based position]

Tube 1: 4x; Tm: 59.80 (59.22-60.76); GC(%): 45.27 (37.04-57.89); PrimerSize: 23.00 (19-27); AmpliconGC(%): 42.73 (27.87-60.00); AmpliconSize: 292.50 (283-300); Penalty: 5.42 (1.29-8.79)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chr1:43554152-43554153,CCGCATCTATGACCTCAACATC,CTGAAGGGGTACAGCGTCT,59.45,59.22,22,19,50.00,57.89,chr1:43553954-43553975,chr1:43554235-43554254,300,60.00,chr1:43553954-43554254,8.7860,chr1,43553975,43554235,Target=chr1:43553976-43554235
T1P2,chr1:55148469-55148470,GACTTGCTGTCAAACTTCAGGTA,TGGAGTTTTATGAGCTGCACAAA,59.39,59.64,23,23,43.48,39.13,chr1:55148341-55148363,chr1:55148605-55148628,287,27.87,chr1:55148341-55148628,7.5790,chr1,55148363,55148605,Target=chr1:55148364-55148605
T1P3,chr1:55914417-55914418,GAGACAAAGGGAAATACTTAAGAACCA,CAGAATACCCGCTTATTTCTGAAGTAT,59.88,59.88,27,27,37.04,37.04,chr1:55914358-55914384,chr1:55914631-55914658,300,41.33,chr1:55914358-55914658,4.0143,chr1,55914384,55914631,Target=chr1:55914385-55914631
T1P4,chr1:55987778-55987779,TTTCTCAAGGCAGGAGGTGAAG,TGCTTTCAAACTGCAGAGCAG,60.76,60.17,22,21,50.00,47.62,chr1:55987672-55987693,chr1:55987934-55987955,283,41.70,chr1:55987672-55987955,1.2913,chr1,55987693,55987934,Target=chr1:55987694-55987934

Designed by MP-Ref