total size: 3 bp, cov size: 3, cov rate: 100.00%, miss size: 0, miss rate: 0.00%, download coverage report.
| Chrom | Start | End | Size (bp) | CovSize (bp) | CovRate (%) | MissSize (bp) | MissRate (%) | |
|---|---|---|---|---|---|---|---|---|
| 1 | chrM | 1000 | 1001 | 1 | 1 | 100.00 | 0 | 0.00 |
| 2 | chrM | 2000 | 2001 | 1 | 1 | 100.00 | 0 | 0.00 |
| 3 | chrM | 3000 | 3001 | 1 | 1 | 100.00 | 0 | 0.00 |
tube: 3, primers: 3, product size range (bp): 182 - 196, download primers.
| ID | TemplateID | FpTm (°G) | RpTm (°G) | FpSize (bp) | RpSize (bp) | AmpSize (bp) | AmpGC (%) |
|---|---|---|---|---|---|---|---|
| T1P1 | chrM:920-1001 | 59.64 | 59.54 | 24 | 25 | 182 | 45.60 |
| ID | TemplateID | FpTm (°G) | RpTm (°G) | FpSize (bp) | RpSize (bp) | AmpSize (bp) | AmpGC (%) |
|---|---|---|---|---|---|---|---|
| T2P1 | chrM:1920-2001 | 59.83 | 59.74 | 20 | 20 | 196 | 45.41 |
| ID | TemplateID | FpTm (°G) | RpTm (°G) | FpSize (bp) | RpSize (bp) | AmpSize (bp) | AmpGC (%) |
|---|---|---|---|---|---|---|---|
| T3P1 | chrM:2920-3001 | 60.42 | 60.74 | 22 | 22 | 195 | 44.62 |
| Target Database | hg38.fa |
| Validation method | sanger |
| Product Size Min Difference | 0 |
| Primer Min Size (bp) | 16 |
| Primer Opt Size (bp) | 22 |
| Primer Max Size (bp) | 32 |
| Primer Min GC (%) | 20 |
| Primer Opt GC (%) | 50 |
| Primer Max GC (%) | 82 |
| Primer Min Tm (°C) | 58 |
| Primer Opt Tm (°C) | 60 |
| Primer Max Tm (°C) | 62 |
| Product Min Size (bp) | 160 |
| Product Max Size (bp) | 200 |
| Product Min GC (%) | 25 |
| Product Opt GC (%) | 50 |
| Product Max GC (%) | 78 |
| Dimer ΔG (kcal/mol) | -3.50 |
| Hairpin Score | 5 |
| Non-specific Amplicon Tm (°C) | 47 |
| Non-specific Amplicon Min Size (bp) | 0 |
| Non-specific Amplicon Max Size (bp) | 300 |
| Min Off-targets | 0 |
| Max Off-targets | 10 |
| Minimum Diffierent Bases | 50 |
| SNP Skip Size | 9 |
| InDel Skip Size | 15 |
| Background Database 1 | - |
| Background Database 2 | - |
| Min Amplicon Count on Background Genomes | 0 |
| Max Amplicon Count on Background Genomes | 0 |