Primer Design Reports (2025-07-21 17:19:59)
[0-based position]

Tube 1: 1x; Tm: 59.59 (59.54-59.64); GC(%): 40.92 (36.00-45.83); PrimerSize: 24.50 (24-25); AmpliconGC(%): 45.60 (45.60-45.60); AmpliconSize: 182.00 (182-182); Penalty: 3.22 (3.22-3.22)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chrM:920-1001,CTAAGCTATACTAACCCCAGGGTT,AAGCCACTTTCGTAGTCTATTTTGT,59.64,59.54,24,25,45.83,36.00,chrM:852-875,chrM:1009-1034,182,45.60,chrM:852-1034,3.2218,chrM,875,1009,Target=chrM:876-1009

Tube 2: 1x; Tm: 59.79 (59.74-59.83); GC(%): 50.00 (50.00-50.00); PrimerSize: 20.00 (20-20); AmpliconGC(%): 45.41 (45.41-45.41); AmpliconSize: 196.00 (196-196); Penalty: 0.93 (0.93-0.93)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T2P1,chrM:1920-2001,TTTGCAAGGAGAGCCAAAGC,AGAGGGTTCTGTGGGCAAAT,59.83,59.74,20,20,50.00,50.00,chrM:1875-1894,chrM:2051-2071,196,45.41,chrM:1875-2071,0.9275,chrM,1894,2051,Target=chrM:1895-2051

Tube 3: 1x; Tm: 60.58 (60.42-60.74); GC(%): 50.00 (50.00-50.00); PrimerSize: 22.00 (22-22); AmpliconGC(%): 44.62 (44.62-44.62); AmpliconSize: 195.00 (195-195); Penalty: 0.82 (0.82-0.82)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T3P1,chrM:2920-3001,TCCGAGCAGTACATGCTAAGAC,GAACCTTTAATAGCGGCTGCAC,60.42,60.74,22,22,50.00,50.00,chrM:2840-2861,chrM:3013-3035,195,44.62,chrM:2840-3035,0.8244,chrM,2861,3013,Target=chrM:2862-3013

Designed by MP-Ref