| Primer ID | Sequence (5' → 3') | Size (bp) | GC (%) | Tm (°C) | ΔG (kcal/mol) | PBN* | MBN* |
|---|---|---|---|---|---|---|---|
| P1 | CCCTGGGCTCTGTAAAGAATAGTG | 24 | 50.00 | 61.23 | -24.52 | 16338 | 16595 |
| P2 | ATCAGAGCTTAAACTGGGAAGCTG | 24 | 45.83 | 61.41 | -24.59 | 38955 | 38976 |
No hairpins found.
No dimers found.
| Amp ID | Product Name | Fp x Rp | Product Size (bp) | Fp Tm (°C) | Rp Tm (°C) | Fp ΔG (kcal/mol) | Rp ΔG (kcal/mol) |
|---|---|---|---|---|---|---|---|
| 1 | chrY | P2 x P1 | 112 | 62.64 | 61.89 | -25.44 | -25.26 |
| 2 | chrX | P1 x P2 | 106 | 61.86 | 62.15 | -25.52 | -25.23 |
Size = 112 bp, GC content = 43.75%
F: Tm = 62.64 °C, ΔG = -25.44 kcal/mol
R: Tm = 61.89 °C, ΔG = -25.26 kcal/mol
Binding sites: 6869847(24/24) ... 6869958(24/24)
>>>P2
1 24
5' ATCAGAGCTTAAACTGGGAAGCTG 3'
:::::::::::::::::::::::: 6869958
5' ATCAGAGCTTAAACTGGGAAGCTGatggtaggaactgta...gatgaagaatccaccCACTATTCTTTACAGAGCCCAGGG 3'
6869847 ::::::::::::::::::::::::
3' CACTATTCTTTACAGAGCCCAGGG 5'
24 1
P1<<<
>Amp_1 P2 + P1 ==> chrY:6869846-6869958
ATCAGAGCTTAAACTGGGAAGCTGatggtaggaactgtaaaattgggaccacttgagaaaccactttatttgggatgaag
aatccaccCACTATTCTTTACAGAGCCCAGGG
Size = 106 bp, GC content = 43.40%
F: Tm = 61.86 °C, ΔG = -25.52 kcal/mol
R: Tm = 62.15 °C, ΔG = -25.23 kcal/mol
Binding sites: 11296874(24/24) ... 11296979(24/24)
>>>P1
1 24
5' CCCTGGGCTCTGTAAAGAATAGTG 3'
:::::::::::::::::::::::: 11296979
5' CCCTGGGCTCTGTAAAGAATAGTGtgttgattctttatc...tacagttcctaccacCAGCTTCCCAGTTTAAGCTCTGAT 3'
11296874 ::::::::::::::::::::::::
3' CAGCTTCCCAGTTTAAGCTCTGAT 5'
24 1
P2<<<
>Amp_2 P1 + P2 ==> chrX:11296873-11296979
CCCTGGGCTCTGTAAAGAATAGTGtgttgattctttatcccagatgtttctcaagtggtcctgattttacagttcctacc
acCAGCTTCCCAGTTTAAGCTCTGAT
| Database | hg38.fa |
| Kvalue | 9 |
| MisMatch | 0 |
| Product Min Size (bp) | 0 |
| Product Max Size (bp) | 200 |
| Tm (°C) | 55 |
| Concentration of monovalent cations (mM) | 50 |
| Concentration of divalent cations (mM) | 1.5 |
| Concentration of dNTPs (mM) | 0.25 |
| Concentration of annealing oligos (nM) | 50 |