Specificity analysis

Number of primer sequences should <= 500?, degenerate primers should <= 2. Otherwise, please use the local command-line version.
Multiple selection supported (<= 5).

Potential amplicon settings

Min
°C
Minimum Tm value required for the binding stability between primer and its binding sites.
From
To
Product size: 0 (min) - 100000 (max).
Use the local command-line version for more flexibility.

Thermodynamic settings

mM
mM
mM
nM

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