MP2: Multiplex PCR primer design result

Done Task link: b4983dfa-14ae-4b30-bfc4-716f7783b5cd (remember for later use)

Coverage report

total size: 7242 bp, cov size: 6981, cov rate: 96.4%, miss size: 261, miss rate: 3.6%, download report.

Chrom Start End Size (bp) CovSize (bp) CovRate (%) MissSize (bp) MissRate (%)
1 chr7 116672185 116672587 402 177 44.03 225 55.97
2 chr7 116699060 116700294 1234 1234 100 0 0
3 chr7 116731657 116731869 212 212 100 0 0
4 chr7 116739939 116740094 155 155 100 0 0
5 chr7 116740841 116741035 194 194 100 0 0
6 chr7 116755344 116755525 181 181 100 0 0
7 chr7 116757426 116757549 123 123 100 0 0
8 chr7 116757627 116757784 157 157 100 0 0
9 chr7 116758448 116758630 182 182 100 0 0
10 chr7 116759380 116759500 120 120 100 0 0
11 chr7 116763039 116763278 239 239 100 0 0
12 chr7 116769634 116769801 167 167 100 0 0
13 chr7 116771487 116771664 177 177 100 0 0
14 chr7 116771838 116771999 161 161 100 0 0
15 chr7 116774870 116775121 251 251 100 0 0
16 chr7 116777378 116777479 101 101 100 0 0
17 chr7 116778765 116778967 202 202 100 0 0
18 chr7 116781977 116782107 130 130 100 0 0
19 chr7 116783293 116783479 186 186 100 0 0
20 chr7 116795644 116795801 157 157 100 0 0
21 chr7 116795876 116798387 2511 2475 98.57 36 1.43

Primer list

tube: 2, primers: 49, product size range (bp): 165 - 260, download primers.

ID Target FpTm (°G) RpTm (°G) FpSize (bp) RpSize (bp) AmpSize (bp) AmpGC (%) FpSeq RpSeq
T1P1 chr7:116795876-116796036 60.78 60.77 23 23 247 44.53 CCTTCAAAGGGTCTCTTACAGCA TCCACCTCATCATCAGCGTTATC
T1P2 chr7:116796249-116796409 60.97 60.74 23 24 259 40.15 TCTTTGCTCTTGCCAAAATTGCA CAAGAAGCCCTCAATATTTCCTGC
T1P3 chr7:116796585-116796745 60.15 61.42 18 18 255 44.31 CCCCAGAACAGGCCACTC tgtgcgcctgtagtccca
T1P4 chr7:116796920-116797080 59.9 60.3 20 28 253 33.2 ctcagcctcccaaagtgcta TCTCAAACAGTGGGTTTTTATATGACAA
T1P5 chr7:116797330-116797490 60.91 61.28 23 22 245 40.82 GGTTCCAATCACAGCTCATAGGT TCACAAAGTTCTGCTCAGGGTG
T1P6 chr7:116797661-116797791 60.71 61.16 23 23 247 33.2 ACTCTAACTGGTTTTGTCGACGT CCTTGGCTTCAAGTCCTGATGAT
T1P7 chr7:116797960-116798120 60.91 61.1 23 23 238 38.66 AGCTCTCTGATAGTGCAGAGACT AGTCCTGTTGATGGGATGTTTCC
T1P8 chr7:116798289-116798387 61.4 61.15 24 24 254 38.98 CTGGACAGATTGTGGGAGTAAGTG CCTACATGGATGAGCTCTATGCAG
T1P9 chr7:116699253-116699413 59.76 61.08 22 22 231 44.16 GGAAACACCCATCCAGAATGTC TAGGTGTCGACAACTAGAGCCA
T1P10 chr7:116699585-116699745 60.36 60.36 22 22 236 44.49 TCACTGCATATTCTCCCCACAG CATCAATGTAGGACTGGTCCGT
T1P11 chr7:116699945-116700105 59.83 61.62 23 20 260 43.85 AGGGAAACTCTAGATGCTCAGAC TCTGGCTTGCTTTGTGCGAA
T1P12 chr7:116700274-116700294 60.97 60.74 20 21 237 39.66 CTGCCGAACCAATGGATCGA AGCACAAAAGAAGCCCTGGAT
T1P13 chr7:116672448-116672587 61.53 60.43 18 22 237 62.03 CCGAGCGCTTTGTgagca AGTCAGGTCTCTTAGGGGAACA
T1P14 chr7:116775056-116775121 60.75 60.54 22 22 225 43.11 ACTGTCCACATTGACCTCAGTG CACTGCTCTGTCAGTTGCTTTC
T1P15 chr7:116763214-116763278 61.45 60.93 27 28 255 37.25 GAGATAATCTGTTGTACCACTCCTTCC GCTATGAAGTCAATTAAGTTTGAGCTGA
T1P16 chr7:116731839-116731869 61.6 61.78 22 23 260 40.77 GCAGCGCGTTGACTTATTCATG TTGGCTTTCAGTCTGTGTTCCAG
T1P17 chr7:116778932-116778967 61.47 60.89 21 22 165 46.06 AATCTGCCTGCGAAGTGAAGG AGAGCAGGCCTATTTTGAAGGG
T1P18 chr7:116783293-116783479 60.35 60.73 28 28 259 37.84 GTAAATTATTCTATTTCAGCCACGGGTA TGAAAGTAAAAGAGGAGAAACTCAGAGA
T1P19 chr7:116755344-116755495 60.13 61.08 30 29 260 36.15 CATTTTGTTTGTTCGTTTTCCATATATGTG GCATTTTAAGATCCTTACGTATTCATCGT
T1P20 chr7:116771487-116771664 60.89 60.26 29 31 259 34.36 ACAAATATCTATCATGGCTAAATGCTGAC GACTGTGTTCTTAAGGTAACTTCTAAAAATG
T1P21 chr7:116771838-116771999 60.25 60.62 24 24 248 40.73 CCCATGATAGCCGTCTTTAACAAG GTCACAACCCACTGAGGTATATGT
T1P22 chr7:116757720-116757784 61.0 61.44 25 24 241 34.85 CCCTCCAGGATCCTGTAATAACAAG CTTCTCCACACACACACACAAAAC
T1P23 chr7:116739939-116740094 59.14 60.75 24 22 233 42.92 AAACTGAGCTTGTTGGAATAAGGA GCATTTGGGAAAAACCACGTCT
T1P24 chr7:116757426-116757528 60.91 60.47 24 30 239 35.98 CAAAGCAGTCAGCTCACCATTTAG CAAACATGATAGGATAGAATCTTCCTTACC
T1P25 chr7:116777378-116777479 60.88 60.99 23 24 248 37.1 GTTACGCAGTGCTAACCAAGTTC TAGGCCCATAATTTCAGTGGTAGC
T2P1 chr7:116796057-116796217 61.0 61.12 22 30 260 40.38 AATGTGTCGCTCCGTATCCTTC TGTCAGATAAGAAATTCCTTAGAATCCAGT
T2P2 chr7:116796430-116796590 60.47 61.17 25 24 252 38.49 GAGTCCAAAACTTGAATTCTGGGTT CCTAATGGGTGAATGACACCATCA
T2P3 chr7:116796740-116796900 61.21 59.66 23 26 260 50.38 gtgatcatagctcactgcaacct CTTCCAACCAAAGGAATGTCTATACA
T2P4 chr7:116797142-116797302 61.04 61.35 23 19 254 33.86 ACCCACTGTTTGAGAATGATGCT AACCTGCCACCGAGACAGA
T2P5 chr7:116797492-116797652 60.75 60.92 24 21 235 34.04 AAAAGGCAATTGAAAATCCCAGCT ACAGTGCAGTGTTTTTGTGGC
T2P6 chr7:116797794-116797954 60.53 60.54 25 27 240 41.25 TGCGATAAGGAAATGTACTGATTGC AACGGCAATATTTGGATTGTAATAGCT
T2P7 chr7:116798122-116798282 61.36 61.14 23 22 240 39.58 ACTGTCTCTACCAGGGTCAAGAG TACCATTCAGTTCAGCTGCAGG
T2P8 chr7:116699060-116699220 61.39 61.23 21 22 251 49.0 AACTGAACTGCTCTCGCCTTG AGTGGCACCAAGGAAAATGTGA
T2P9 chr7:116699426-116699586 60.03 61.49 22 21 259 49.03 CACCCAGATTGTTTCCCATGTC TCACCACACAGTCAGGACACT
T2P10 chr7:116699761-116699921 60.08 61.15 23 22 250 39.6 CAGTGAGAAGGCTAAAGGAAACG CACTCCAGAGGCATTTCCATGT
T2P11 chr7:116700109-116700269 61.7 60.53 22 23 233 44.21 AGACAAATAGGAGCCAGCCTGA AGTGGGGAACTGATGTGACTTAC
T2P12 chr7:116672185-116672195 60.55 62.24 22 18 242 69.83 GGCGAGAAAACTTCTCCACCTA ACCAGACTGAGGCGCTCG
T2P13 chr7:116774870-116775061 61.26 61.26 29 20 260 44.23 CCCATTAAATGAGGTTTTACTGTTGTTCT CACAATCAGGCTACTGGGCC
T2P14 chr7:116763039-116763218 60.87 59.24 21 22 256 40.23 TGCTATGGATGTTGCCAAGCT TTGCCCATTGAGATCATCACTG
T2P15 chr7:116731657-116731839 60.91 60.91 24 24 259 43.63 CAGCTTGTTCATGTCTGGATTCAC CAGAAAGCACTTACCTGCATGAAG
T2P16 chr7:116778765-116778937 60.25 60.95 28 27 238 44.12 CTGTTCCATAATGAAGTTAATGTCTCCA CAGTCAACTTACATGAGTCTCATTTCG
T2P17 chr7:116740841-116741035 60.72 60.61 23 24 258 51.16 CTAGATACCCCTCTGGAAGCTCT ccaaacaaaaaCATGTATGCCAGC
T2P18 chr7:116758448-116758630 61.36 61.41 32 32 260 34.62 TGTGTATCTCTAATAGCTAAAATTCACTTCCT CATCCTTGTATTCATTGTTTTCTTATACCCAT
T2P19 chr7:116755494-116755525 61.36 61.36 22 22 203 42.36 TTCCCAAATAGTGCACCCCTTG CCTATTTTCCAAGCACACCCCA
T2P20 chr7:116769634-116769801 61.21 60.8 24 27 258 39.15 CCATTGTTAGCATTCCTGCAGAAC CACTGGCTTCCTTATTTACATCATGAG
T2P21 chr7:116757627-116757724 61.33 59.23 22 25 253 35.57 CCACGGGACAACACAATACAGT TTCCACCAATTGAAATGTGTCTAGA
T2P22 chr7:116795644-116795801 60.49 60.88 22 22 236 45.76 TCTCTCACCTCATCTGTCCTGT CCTTTGAAGGCAGGCATTTCTG
T2P23 chr7:116781977-116782107 59.5 60.67 27 24 241 38.59 AGAACAGTAGATGCTTAGTTTATGCTT GCTTAACTAGCATTGAACAGTGGG
T2P24 chr7:116759380-116759500 60.55 61.35 22 22 239 42.68 gacctgtaatcagtgcaggtga TGCAGAGCTCTGAGTAGAACCA

Parameters

Target Database hg38.fa
Background Database 1 -
Background Database 2 -
Primer Min Tm (°C) 59.0
Primer Max Tm (°C) 63.0
Product Min Size (bp) 160
Product Max Size (bp) 260
Dimer Score 5
Dimer ΔG -3.5
Hairpin Score 5
Tm (°C) 57.0
Specificity Min Size (bp) 0
Specificity Max Size (bp) 350