MP2: Enhanced Multiplex PCR Primer Design for Versatile Applications

Introduction Parameters help Examples

Target settings

: primers should be chosen around, not within, the regions.

Primer settings

°C
°C
°C
The length at the 3' end without any SNPs or InDels.
kcal/mol

Product settings

Leave 0 for automatically get the tube number.
NGS or Gel electrophoresis
bp
Only for size discrimination method, e.g., Gel electrophoresis.

Nonspecific analysis settings for target database

Min
°C
Min Tm value required for the binding stability between primer and its binding sites.
Min
Max
bp
Product size: 0 (min) - 1000000 (max).
In practice, allowing up to 10 non-specific amplicons has proven effective based on our experience.
The difference is the edit distance between the target and non-target amplicons.

Nonspecific analysis settings for background databases

Checking amplicons for this genome.
Checking amplicons for this genome.
Min
Max

mM
mM
mM
nM