MFE
primer
4.0
PCR Primer Quality Control and Design Tools
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Dimer analysis
* Primer sequences (<= 200) (
FASTA format
or just plain sequences one per line,
example
)
Allowed max mismatches for a dimer
Max
Only output dimers with 3' end binded
Yes
No
Score
Min
Score cutoff value to filter dimers
ΔG
Max
kcal/mol
ΔG cutoff value to filter dimers
Advanced settings
Concentration of monovalent cations (usually KCl)
mM
Concentration of divalent cations (usually MgCl
2
)
mM
Concentration of dNTPs
mM
Annealing oligo concentration
nM
Run
Quality Control
Coverage analysis for Microbe
New feature
Specificity analysis
Improvement
Dimer analysis
Hairpin analysis
Primer Design
Singleplex / Multiplex PCR
New feature
PCR for Diverse Genomes
In progress
Contact
For bug reports or suggestions, please
email
us.
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