Since its launch in 2009, the MFEprimer server has been continuously operating for 16 years. We remain committed to maintaining this platform as a sustainable, long-term resource for the global research community.
From version 4.0, the MFEprimer Web Server serves as a comprehensive platform for PCR primer quality control and design tools. We have recently launched a new feature, the MP-Ref, which is a multiplex PCR primer design tool based on reference genome. Additionally, we are developing a primer design project aimed at diverse genomes. Below is a detailed list of our tools: