Primer Design Reports (2025-07-22 11:39:50)
[0-based position]

Tube 1: 21x; Tm: 60.02 (59.19-61.13); GC(%): 49.43 (43.48-52.38); PrimerSize: 21.93 (20-23); AmpliconGC(%): 42.19 (35.54-54.07); AmpliconSize: 904.33 (702-1000); Penalty: 2.22 (0.23-6.37)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chr5:70924941-70925441,cacgcctatagtcctagctactg,gaactcctgagaaacctgctcta,60.08,60.02,23,23,52.17,47.83,chr5:70924729-70924751,chr5:70925690-70925713,984,54.07,chr5:70924729-70925713,6.3738,chr5,70924751,70925690,
T1P2,chr5:70926378-70926878,gaggcaggagaatgtcatgaag,tggtctccttcaactcatgacc,59.31,60.22,22,22,50.00,50.00,chr5:70926175-70926196,chr5:70927137-70927159,984,45.12,chr5:70926175-70927159,2.8740,chr5,70926196,70927137,
T1P3,chr5:70927684-70928184,agcctggacgatatagtgagac,aaccatcaccttctaggctctg,59.37,60.03,22,22,50.00,50.00,chr5:70927578-70927599,chr5:70928402-70928424,846,48.11,chr5:70927578-70928424,3.6157,chr5,70927599,70928402,
T1P4,chr5:70929104-70929604,ctggggatcacaattcagcatg,ctgaggatgccttttcctttcg,60.16,60.09,22,22,50.00,50.00,chr5:70928858-70928879,chr5:70929809-70929831,973,49.33,chr5:70928858-70929831,4.0447,chr5,70928879,70929809,
T1P5,chr5:70930645-70931145,ggctttttccagtttgggactg,caagcccaggcaacataatgag,60.75,60.42,22,22,50.00,50.00,chr5:70930591-70930612,chr5:70931418-70931440,849,37.81,chr5:70930591-70931440,0.6869,chr5,70930612,70931418,
T1P6,chr5:70931990-70932490,gcaagcttcacatacgtaaggt,gatgggtccattcagttggttg,59.51,60.03,22,22,45.45,50.00,chr5:70931923-70931944,chr5:70932901-70932923,1000,42.50,chr5:70931923-70932923,2.1893,chr5,70931944,70932901,
T1P7,chr5:70933633-70934133,gcctttcctgacagaactcatg,ATCCTCAGAgagcctctaagca,59.77,60.63,22,22,50.00,50.00,chr5:70933261-70933282,chr5:70934173-70934195,934,40.58,chr5:70933261-70934195,1.3047,chr5,70933282,70934173,
T1P8,chr5:70934777-70935277,CAGGACCACCCTTCATTTTCTAC,catcttggcaaaactggtctcg,59.51,60.61,23,22,47.83,50.00,chr5:70934641-70934663,chr5:70935549-70935571,930,47.74,chr5:70934641-70935571,4.3257,chr5,70934663,70935549,
T1P9,chr5:70936063-70936563,agcgagactcttgtctcaagag,gaagttgaggatgcagtgagtg,60.03,59.77,22,22,50.00,50.00,chr5:70935736-70935757,chr5:70936699-70936721,985,38.17,chr5:70935736-70936721,0.2273,chr5,70935757,70936699,
T1P10,chr5:70937301-70937801,gtcctggactcaagcaattcac,tgacagaggaagaccctgtcta,60.03,60.16,22,22,50.00,50.00,chr5:70937165-70937186,chr5:70937845-70937867,702,40.88,chr5:70937165-70937867,1.0399,chr5,70937186,70937845,
T1P11,chr5:70938479-70938979,GGAAAGCAGGAAAGGGAAATGG,gcaattctcctgcctcagtgt,60.29,61.13,22,21,50.00,52.38,chr5:70938338-70938359,chr5:70939316-70939337,999,35.54,chr5:70938338-70939337,1.9494,chr5,70938359,70939316,
T1P12,chr5:70939807-70940307,gttggccaacatcatcaccttc,ACTGTCTGCAAACACACTTCTG,60.61,59.83,22,22,50.00,45.45,chr5:70939754-70939775,chr5:70940640-70940662,908,41.08,chr5:70939754-70940662,2.0453,chr5,70939775,70940640,
T1P13,chr5:70941190-70941690,AGAGAGGTTGAGAGCAAGGAGA,tagcgggagggagataaaatgc,60.76,60.49,22,22,50.00,50.00,chr5:70941156-70941177,chr5:70942059-70942081,925,37.30,chr5:70941156-70942081,0.7940,chr5,70941177,70942059,
T1P14,chr5:70942802-70943302,AGTTTGATCCACCGAGGCAT,TGAGAGCGCCTCAGGATTTT,59.90,59.90,20,20,50.00,50.00,chr5:70942639-70942658,chr5:70943579-70943599,960,39.69,chr5:70942639-70943599,1.0369,chr5,70942658,70943579,
T1P15,chr5:70944197-70944697,tcagctcactgcaacttcca,TGGTCCAGAAGGAAATGGAGG,60.04,59.89,20,21,50.00,52.38,chr5:70943855-70943874,chr5:70944729-70944750,895,37.88,chr5:70943855-70944750,0.9568,chr5,70943874,70944729,
T1P16,chr5:70945322-70945822,ctcctgggttcaagcaattctg,GAGAATCTGGACATATGGGAGGT,60.03,59.63,22,23,50.00,47.83,chr5:70945216-70945237,chr5:70946079-70946102,886,40.18,chr5:70945216-70946102,1.1583,chr5,70945237,70946079,
T1P17,chr5:70946830-70947330,CACAAAGATCTGGGGTAATGTGT,caacctctgcctcccagattaa,59.19,60.29,23,22,43.48,50.00,chr5:70946801-70946823,chr5:70947604-70947626,825,44.61,chr5:70946801-70947626,3.3200,chr5,70946823,70947604,
T1P18,chr5:70948246-70948746,AGTCACGCTTCAGTTGTTGTAG,cagtactggtgcattctacagc,59.38,59.58,22,22,45.45,50.00,chr5:70947987-70948008,chr5:70948812-70948834,847,39.67,chr5:70947987-70948834,1.2966,chr5,70948008,70948812,
T1P19,chr5:70949531-70950031,GTGGTTGAGATAGGCTCTGAGT,GATGTTCCTGAGCCCGCTTATA,59.76,60.49,22,22,50.00,50.00,chr5:70949363-70949384,chr5:70950086-70950108,745,40.27,chr5:70949363-70950108,1.1420,chr5,70949384,70950086,
T1P20,chr5:70950572-70951072,caggagagttgcttgaactgag,tgagcaatagagtgagacctgg,59.45,59.76,22,22,50.00,50.00,chr5:70950321-70950342,chr5:70951115-70951137,816,48.77,chr5:70950321-70951137,3.8204,chr5,70950342,70951115,
T1P21,chr5:70951952-70952452,gagctcaggtgatccaactgt,tccagatctgtctgatcgtttct,60.24,59.51,21,23,52.38,43.48,chr5:70951502-70951522,chr5:70952477-70952500,998,36.77,chr5:70951502-70952500,2.5182,chr5,70951522,70952477,

Tube 2: 21x; Tm: 59.96 (59.05-60.56); GC(%): 49.07 (40.00-55.00); PrimerSize: 21.98 (20-25); AmpliconGC(%): 41.10 (34.80-48.70); AmpliconSize: 903.57 (721-999); Penalty: 2.02 (0.42-4.71)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T2P1,chr5:70925680-70926180,aggggaaccacttaggagtct,taggatcatgccactgtactcc,60.10,59.56,21,22,52.38,50.00,chr5:70925488-70925508,chr5:70926388-70926410,922,48.70,chr5:70925488-70926410,4.4800,chr5,70925508,70926388,
T2P2,chr5:70927127-70927627,gagccactgcacTaagatagga,tgctgggattacaggtgtcag,59.83,60.24,22,21,50.00,52.38,chr5:70926716-70926737,chr5:70927694-70927715,999,46.05,chr5:70926716-70927715,3.4952,chr5,70926737,70927694,
T2P3,chr5:70928392-70928892,ggatcaatgtactgccagtcct,cttgtcaccagactcacagtct,60.36,60.16,22,22,50.00,50.00,chr5:70928331-70928352,chr5:70929114-70929136,805,43.48,chr5:70928331-70929136,2.0865,chr5,70928352,70929114,
T2P4,chr5:70929799-70930299,gtgtgtggaggagaaaagagga,gatctgccagccttggcTATAT,60.16,60.56,22,22,50.00,50.00,chr5:70929763-70929784,chr5:70930655-70930677,914,41.25,chr5:70929763-70930677,1.6158,chr5,70929784,70930655,
T2P5,chr5:70931408-70931908,caaacacctggtatggtcagtc,gcttccagcttccttatctgag,59.44,59.05,22,22,50.00,50.00,chr5:70931044-70931065,chr5:70932000-70932022,978,40.49,chr5:70931044-70932022,1.4043,chr5,70931065,70932000,
T2P6,chr5:70932891-70933391,cacagggctatttctgactctca,caacaagagcgaaactcttgtct,60.34,59.96,23,23,47.83,43.48,chr5:70932760-70932782,chr5:70933643-70933666,906,37.97,chr5:70932760-70933666,1.3975,chr5,70932782,70933643,
T2P7,chr5:70934163-70934663,ccctggtcatatcttggttgga,cacacctgtagtcccagatact,60.02,59.16,22,22,50.00,50.00,chr5:70933953-70933974,chr5:70934787-70934809,856,38.79,chr5:70933953-70934809,0.8070,chr5,70933974,70934787,
T2P8,chr5:70935539-70936039,agctgggactacagatgtgaac,gagtttcaggttgcaagacgaa,60.29,59.90,22,22,50.00,45.45,chr5:70935289-70935310,chr5:70936073-70936095,806,38.96,chr5:70935289-70936095,0.8558,chr5,70935310,70936073,
T2P9,chr5:70936689-70937189,ctccatgttgaggctggtct,tgcactacagcctgagagac,59.90,59.62,20,20,55.00,55.00,chr5:70936342-70936361,chr5:70937311-70937331,989,47.22,chr5:70936342-70937331,4.7122,chr5,70936361,70937311,
T2P10,chr5:70937835-70938335,CGGTTTCCTCCATATGATCTTGC,CTAGACTACGGGAATGCTGTTCT,60.02,60.15,23,23,47.83,47.83,chr5:70937577-70937599,chr5:70938489-70938512,935,38.82,chr5:70937577-70938512,0.9931,chr5,70937599,70938489,
T2P11,chr5:70939306-70939806,GACATTTGGGATGATACAGCACTG,ggtagtctggactattgatgaccc,60.49,60.50,24,24,45.83,50.00,chr5:70938850-70938873,chr5:70939817-70939841,991,38.04,chr5:70938850-70939841,1.5751,chr5,70938873,70939817,
T2P12,chr5:70940630-70941130,GAGATTGAAATGGGGCTCGATG,GCATCATACACCTGACATCAGC,59.96,59.97,22,22,50.00,50.00,chr5:70940379-70940400,chr5:70941200-70941222,843,42.94,chr5:70940379-70941222,1.6741,chr5,70940400,70941200,
T2P13,chr5:70942049-70942549,TTACCAAGGGGGaagagagcta,TGGTGGAGGGAGAAAAGAGTTC,60.50,60.16,22,22,50.00,50.00,chr5:70941986-70942007,chr5:70942812-70942834,848,38.09,chr5:70941986-70942834,0.4174,chr5,70942007,70942812,
T2P14,chr5:70943569-70944069,gagcttgcagtgagtcaagatc,CTGAGACCAATGAAGCATGATGT,59.84,59.58,22,23,50.00,43.48,chr5:70943378-70943399,chr5:70944207-70944230,852,39.20,chr5:70943378-70944230,1.3326,chr5,70943399,70944207,
T2P15,chr5:70944719-70945219,TCTGTTTGACTTCAGGATTTGGTAC,tcaggagttcaagatcagtctgg,59.77,60.02,25,23,40.00,47.83,chr5:70944492-70944516,chr5:70945332-70945355,863,40.32,chr5:70944492-70945355,2.9290,chr5,70944516,70945332,
T2P16,chr5:70946069-70946569,gcaagacctcgtctttgtttagg,GACAAGTTTGAAGCCATACTCCC,60.33,60.08,23,23,47.83,47.83,chr5:70945906-70945928,chr5:70946840-70946863,957,34.80,chr5:70945906-70946863,2.1187,chr5,70945928,70946840,
T2P17,chr5:70947594-70948094,acatgcctgtaatccgagct,tgtgcccagccaacttgtat,59.68,60.41,20,20,50.00,50.00,chr5:70947555-70947574,chr5:70948256-70948276,721,41.75,chr5:70947555-70948276,1.9028,chr5,70947574,70948256,
T2P18,chr5:70948802-70949302,ggcagtcagtacactttctgtc,CAGTGGCTTCCCACTTCATTAC,59.45,59.77,22,22,50.00,50.00,chr5:70948681-70948702,chr5:70949541-70949563,882,39.23,chr5:70948681-70949563,0.5703,chr5,70948702,70949541,
T2P19,chr5:70950076-70950576,AGACAGAGGACATTTGGGCTAG,gcatatggctgtaatcacagct,60.03,59.37,22,22,50.00,45.45,chr5:70949613-70949634,chr5:70950582-70950604,991,46.32,chr5:70949613-70950604,3.5333,chr5,70949634,70950582,
T2P20,chr5:70951105-70951605,tgcaaggcattacaggcatg,AGGAATGTGAGCACCTTCCTT,59.69,59.82,20,21,50.00,47.62,chr5:70951036-70951055,chr5:70951962-70951983,947,43.08,chr5:70951036-70951983,2.6080,chr5,70951055,70951962,
T2P21,chr5:70952467-70952967,atttgcaggaaatgctggcat,cgaatcacttgagggcagga,59.96,60.26,21,20,42.86,55.00,chr5:70952430-70952450,chr5:70953380-70953400,970,37.63,chr5:70952430-70953400,2.0061,chr5,70952450,70953380,

Designed by MP-Ref