Primer Design Reports (2025-07-21 21:39:00)
[0-based position]

Tube 1: 3x; Tm: 59.93 (59.54-60.49); GC(%): 49.60 (47.62-50.00); PrimerSize: 21.17 (20-22); AmpliconGC(%): 44.56 (43.45-45.41); AmpliconSize: 232.67 (196-290); Penalty: 0.89 (0.67-1.08)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chrM:1000-1001,AAGTCAATAGAAGCCGGCGTA,ACAGGCTCCTCTAGAGGGATAT,60.04,59.54,21,22,47.62,50.00,chrM:917-937,chrM:1185-1207,290,43.45,chrM:917-1207,1.0810,chrM,937,1185,Target=chrM:938-1185
T1P2,chrM:2000-2001,TTTGCAAGGAGAGCCAAAGC,AGAGGGTTCTGTGGGCAAAT,59.83,59.74,20,20,50.00,50.00,chrM:1875-1894,chrM:2051-2071,196,45.41,chrM:1875-2071,0.9275,chrM,1894,2051,Target=chrM:1895-2051
T1P3,chrM:3000-3001,CTAGGGATAACAGCGCAATCCT,TCTCTTGTCCTTTCGTACAGGG,60.49,59.96,22,22,50.00,50.00,chrM:2928-2949,chrM:3118-3140,212,44.81,chrM:2928-3140,0.6680,chrM,2949,3118,Target=chrM:2950-3118

Designed by MP-Ref