Primer Design Reports (2025-07-19 10:55:26)
[0-based position]

Tube 1: 64x; Tm: 59.93 (58.12-61.42); GC(%): 43.86 (25.00-62.50); PrimerSize: 23.81 (16-32); AmpliconGC(%): 37.44 (27.14-64.79); AmpliconSize: 261.38 (180-280); Penalty: 5.90 (0.81-29.07)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chr13:32889611-32889809,TGCGTGTGCTGCGTGT,TCGTCCCAACCCACTACC,60.70,58.70,16,18,62.50,61.11,chr13:32889567-32889582,chr13:32889816-32889834,267,64.79,chr13:32889567-32889834,11.9557,chr13,32889582,32889816,
T1P2,chr13:32893208-32893407,GTCACAAATTTGTCTGTCACTGGT,TTCTTTTACAGGAGATTGGTACAGC,60.43,59.59,24,25,41.67,40.00,chr13:32893157-32893180,chr13:32893412-32893437,280,35.71,chr13:32893157-32893437,15.3458,chr13,32893180,32893412,
T1P3,chr13:32899207-32899326,ataatcCAGAGTATATACATTCTCACTGA,CTTCTACCAGGCTCTTAGCCAA,58.23,60.03,29,22,31.03,50.00,chr13:32899165-32899193,chr13:32899415-32899437,272,30.51,chr13:32899165-32899437,9.8762,chr13,32899193,32899415,Target=chr13:32899194-32899415
T1P4,chr13:32900379-32900424,ACACCACAAAGAGATAAGTCAGGT,CTGAATTGCCTGTATGAGGCAG,60.19,59.90,24,22,41.67,50.00,chr13:32900265-32900288,chr13:32900519-32900541,276,32.25,chr13:32900265-32900541,5.2341,chr13,32900288,32900519,Target=chr13:32900289-32900519
T1P5,chr13:32903574-32903634,AGATGTGCTTTTTGATGTCTGACA,AGGACCAGGTTTAGAGACTTTCTC,59.71,60.01,24,24,37.50,45.83,chr13:32903495-32903518,chr13:32903744-32903768,273,28.57,chr13:32903495-32903768,7.1396,chr13,32903518,32903744,Target=chr13:32903519-32903744
T1P6,chr13:32906403-32906563,ACTGTTTCTATGAGAAAGGTTGTGAG,CCTAGTCTTGCTAGTTCTTACTTTTTGT,59.83,60.14,26,28,38.46,35.71,chr13:32906322-32906347,chr13:32906574-32906602,280,29.29,chr13:32906322-32906602,8.9889,chr13,32906347,32906574,
T1P7,chr13:32906722-32906882,GTGGAACCAAATGATACTGATCCA,ACGTGGCAAAGAATTCTCTGAAG,59.34,60.02,24,23,41.67,43.48,chr13:32906686-32906709,chr13:32906936-32906959,273,38.46,chr13:32906686-32906959,3.8789,chr13,32906709,32906936,
T1P8,chr13:32907082-32907242,GAGAGATGAAGAGCAGCATCTTG,GAGTCCTCCTTCTGTGAGCAAA,59.71,60.49,23,22,47.83,50.00,chr13:32907015-32907037,chr13:32907248-32907270,255,38.82,chr13:32907015-32907270,2.4958,chr13,32907037,32907248,
T1P9,chr13:32907449-32907529,CCACCACACAGAATTCTGTAGC,ACACAGAAGGAATCGTCATCTATAA,59.77,58.18,22,25,50.00,36.00,chr13:32907311-32907332,chr13:32907565-32907590,279,31.54,chr13:32907311-32907590,7.5427,chr13,32907332,32907565,
T1P10,chr13:32910592-32910752,CTCTCAGGATCTTGATTATAAAGAAGCAA,GGAAGTAGGAGTTAAAATAAGAGTGCTG,60.13,60.51,29,28,34.48,39.29,chr13:32910537-32910565,chr13:32910789-32910817,280,36.43,chr13:32910537-32910817,6.3525,chr13,32910565,32910789,
T1P11,chr13:32910982-32911142,TGGAAAAGAATCAAGATGTATGTGCT,CCTTTCATTAGCTACTTGGAAGACAA,59.88,59.89,26,26,34.62,38.46,chr13:32910935-32910960,chr13:32911160-32911186,251,31.87,chr13:32910935-32911186,7.4976,chr13,32910960,32911160,
T1P12,chr13:32911321-32911481,GGAGACACAGGTGATAAACAAGC,CTGAAGCTACCTCCAAAACTGTG,60.08,60.02,23,23,47.83,47.83,chr13:32911282-32911304,chr13:32911498-32911521,239,34.31,chr13:32911282-32911521,3.8091,chr13,32911304,32911498,
T1P13,chr13:32911651-32911811,AGCTTGTGTTGAAATTGTAAATACCTTG,CTGAGTAAATTCAAACTGACTTCCTGA,60.15,60.10,28,27,32.14,37.04,chr13:32911617-32911644,chr13:32911852-32911879,262,32.44,chr13:32911617-32911879,8.1315,chr13,32911644,32911852,
T1P14,chr13:32912005-32912165,GACCACTTCTGAGGAATGCAGA,TCACAGCTTTTTGCAGAGCTTC,60.55,60.48,22,22,50.00,45.45,chr13:32911950-32911971,chr13:32912185-32912207,257,40.86,chr13:32911950-32912207,1.5335,chr13,32911971,32912185,
T1P15,chr13:32912380-32912540,TCTGTTGTTTCAATGTTTAAGATAGAAAATCA,GATCAGTAAATAGCAAGTCCGTTTCA,60.01,60.22,32,26,25.00,38.46,chr13:32912293-32912324,chr13:32912547-32912573,280,27.14,chr13:32912293-32912573,22.0440,chr13,32912324,32912547,
T1P16,chr13:32912708-32912868,GCGAAAGCTCAAGAAGCATGTC,CTGTTTCCTCATAACTTAGAATGTCCA,61.24,59.72,22,27,50.00,37.04,chr13:32912668-32912689,chr13:32912915-32912942,274,31.02,chr13:32912668-32912942,7.5701,chr13,32912689,32912915,
T1P17,chr13:32913084-32913244,AACTAGTGACCTTCCAGGGACA,GCATTTCTTTACACTTTGGGGCA,60.97,60.78,22,23,50.00,43.48,chr13:32912996-32913017,chr13:32913252-32913275,279,40.50,chr13:32912996-32913275,2.3807,chr13,32913017,32913252,
T1P18,chr13:32913421-32913581,TGTAGACAAACTGAAAATCTCAAAACATC,TCTGCAGTATTTATTCTTTCTGGTTGAC,59.93,60.67,29,28,31.03,35.71,chr13:32913352-32913380,chr13:32913590-32913618,266,31.95,chr13:32913352-32913618,8.8366,chr13,32913380,32913590,
T1P19,chr13:32913735-32913895,ACAAAAATCATCTCTCCGAAAAACAAG,GCTAGTCACAAGTTCCTCAACG,59.89,59.77,27,22,33.33,50.00,chr13:32913674-32913700,chr13:32913918-32913940,266,32.33,chr13:32913674-32913940,7.3532,chr13,32913700,32913918,
T1P20,chr13:32914065-32914225,CCTGCATTTAGGATAGCCAGTG,CACTCTGAATGTCAGCAAAAACC,59.37,59.22,22,23,50.00,43.48,chr13:32914015-32914036,chr13:32914248-32914271,256,33.98,chr13:32914015-32914271,5.1213,chr13,32914036,32914248,
T1P21,chr13:32914416-32914576,CTTCATAAGTCAGTCTCATCTGCAA,TCCAGAGAAAGCAGATGAATTTACC,59.43,59.65,25,25,40.00,40.00,chr13:32914384-32914408,chr13:32914638-32914663,279,35.84,chr13:32914384-32914663,5.5164,chr13,32914408,32914638,
T1P22,chr13:32914797-32914957,TCAGAACTGAGCATAGTCTTCACT,CAACAAGTGACACTTTGGTTCCT,59.77,60.08,24,23,41.67,43.48,chr13:32914748-32914771,chr13:32914992-32915015,267,33.71,chr13:32914748-32915015,5.3219,chr13,32914771,32914992,
T1P23,chr13:32915189-32915338,TTTGAAACAGAAGCAGTAGAAATTGC,GTGATTGGCAACACGAAAGGTAA,59.83,60.52,26,23,34.62,43.48,chr13:32915158-32915183,chr13:32915347-32915370,212,37.26,chr13:32915158-32915370,6.2268,chr13,32915183,32915347,
T1P24,chr13:32920958-32921038,ACATTTATTGAGCATCTGTTACATTCAC,AACGAGACTTTTCTCATACTGTATTAGAA,59.63,59.37,28,29,32.14,31.03,chr13:32920827-32920854,chr13:32921059-32921088,261,28.35,chr13:32920827-32921088,10.8444,chr13,32920854,32921059,Target=chr13:32920855-32921059
T1P25,chr13:32929166-32929326,TTTAGCAGTTTCAGGACATCCAT,GCTACTGCTTGATTGGAGTTGT,58.60,59.44,23,22,39.13,45.45,chr13:32929111-32929133,chr13:32929368-32929390,279,33.69,chr13:32929111-32929390,6.3714,chr13,32929133,32929368,
T1P26,chr13:32930559-32930751,ccAGGGGTTGTGCTTTTTAAATTTC,TGTCATAAAAGCCATCAGTATTGTAGAC,60.30,60.30,25,28,40.00,35.71,chr13:32930504-32930528,chr13:32930756-32930784,280,38.93,chr13:32930504-32930784,15.6111,chr13,32930528,32930756,Target=chr13:32930529-32930756
T1P27,chr13:32932053-32932071,TACAGTTGGCTGATGGTGGATG,TCCAGTTCATTAAACCCCAGGAC,60.88,60.79,22,23,50.00,47.83,chr13:32931996-32932017,chr13:32932241-32932264,268,35.82,chr13:32931996-32932264,3.3766,chr13,32932017,32932241,
T1P28,chr13:32937310-32937470,GTGGAATTCTAGAGTCACACTTCC,AGGATCTAACTGGGCCTTAACAG,59.35,60.08,24,23,45.83,47.83,chr13:32937261-32937284,chr13:32937518-32937541,280,36.07,chr13:32937261-32937541,3.9908,chr13,32937284,32937518,
T1P29,chr13:32944533-32944669,GGCAGTTCTAGAAGAATGAAAACTCT,CATCATACCTGTATAGGGTATGCTCT,59.89,59.94,26,26,38.46,42.31,chr13:32944444-32944469,chr13:32944676-32944702,258,36.05,chr13:32944444-32944702,5.1966,chr13,32944469,32944676,
T1P30,chr13:32945087-32945242,gcctggccTGATACAATTAACTTG,AGTCTCTAAGACTTTGTTCTCATATTAGA,60.74,58.49,24,29,45.83,31.03,chr13:32945021-32945044,chr13:32945269-32945298,277,34.30,chr13:32945021-32945298,8.5221,chr13,32945044,32945269,Target=chr13:32945045-32945269
T1P31,chr13:32953448-32953627,TTTTGTTCTGATTGCTTTTTATTCCAA,AAGCATTTACATACTTACCTGAATCTTT,58.12,58.39,27,28,25.93,28.57,chr13:32953408-32953434,chr13:32953642-32953670,262,35.88,chr13:32953408-32953670,10.7832,chr13,32953434,32953642,
T1P32,chr13:32953881-32954055,CACTTCTTCCATTGCATCTTTCTCA,TGGAGATTCCATAAACTAACAAGCA,60.35,59.05,25,25,40.00,36.00,chr13:32953846-32953870,chr13:32954088-32954113,267,33.33,chr13:32953846-32954113,7.7793,chr13,32953870,32954088,Target=chr13:32953871-32954088
T1P33,chr13:32968820-32969015,TGCATCTTAAAATTCATCTAACACATCTATAA,GTCTCTTGAAAGTGGCCCTCTT,59.23,60.76,32,22,25.00,50.00,chr13:32968763-32968794,chr13:32969020-32969042,279,36.92,chr13:32968763-32969042,29.0719,chr13,32968794,32969020,
T1P34,chr13:32971029-32971186,GGGTTTGCAATTTATAAAGCAGCT,CAACAGAATATACGATGGCCTCC,59.34,59.52,24,23,37.50,47.83,chr13:32970991-32971014,chr13:32971224-32971247,256,37.50,chr13:32970991-32971247,4.5729,chr13,32971014,32971224,Target=chr13:32971015-32971224
T1P35,chr13:32972478-32972638,TGCAAAAAGAGAAGAGCCTTGG,GAACCAGACAAAAGAGCTTGGG,59.89,60.22,22,22,45.45,50.00,chr13:32972442-32972463,chr13:32972695-32972717,275,39.27,chr13:32972442-32972717,1.8979,chr13,32972463,32972695,
T1P36,chr13:32972840-32973000,CTCAGACTGAAACGACGTTGTAC,CGATTTTTGCCCGATACACAAAC,60.09,59.90,23,23,47.83,43.48,chr13:32972787-32972809,chr13:32973041-32973064,277,35.74,chr13:32972787-32973064,3.6009,chr13,32972809,32973041,
T1P37,chr13:32973188-32973348,AGAAAAACATCTTTggctgagct,ACTGTACTAAGAGACCTCATTCCA,59.63,58.96,23,24,39.13,41.67,chr13:32973141-32973163,chr13:32973389-32973413,272,39.34,chr13:32973141-32973413,4.5124,chr13,32973163,32973389,
T1P38,chr13:32973556-32973716,GGTTCTGCTATAGTTCCATCCTGT,TCTCAAGTTTGAGTTTGGATGACC,60.44,59.71,24,24,45.83,41.67,chr13:32973514-32973537,chr13:32973755-32973779,265,30.19,chr13:32973514-32973779,6.6362,chr13,32973537,32973755,
T1P39,chr17:41196306-41196466,AAAGTCGGCTGGCCTAAGTC,TTCCTTTGCTCCCTGTTGCT,60.55,60.34,20,20,55.00,50.00,chr17:41196271-41196290,chr17:41196527-41196547,276,41.30,chr17:41196271-41196547,1.7811,chr17,41196290,41196527,
T1P40,chr17:41196698-41196858,AAGAGGAATGGATTATATACCAGAGCT,gttgcagtgagccaagatcatac,59.88,60.46,27,23,37.04,47.83,chr17:41196639-41196665,chr17:41196879-41196902,263,34.98,chr17:41196639-41196902,5.8755,chr17,41196665,41196879,
T1P41,chr17:41197026-41197186,caccatgaccggctaatttctg,AGTCAGAATAGTCCTTGGGCAG,60.16,60.03,22,22,50.00,50.00,chr17:41196971-41196992,chr17:41197193-41197215,244,48.77,chr17:41196971-41197215,2.2443,chr17,41196992,41197193,
T1P42,chr17:41197393-41197553,CTCTTGAGGCCAAGCCACT,AGTCCTTCTACTGTCCTGGCTA,60.13,60.23,19,22,57.89,50.00,chr17:41197323-41197341,chr17:41197581-41197603,280,41.07,chr17:41197323-41197603,2.4755,chr17,41197341,41197581,
T1P43,chr17:41197740-41197824,TGCAGTCAGTAGTGGCTGTG,TGGAGTCGATTGATTAGAGCCT,60.47,59.23,20,22,55.00,45.45,chr17:41197689-41197708,chr17:41197871-41197893,204,52.94,chr17:41197689-41197893,4.7019,chr17,41197708,41197871,
T1P44,chr17:41201132-41201216,TATTGTGTCCTCCCTCTCTGAC,GCTATAAGCCTTCATCCGGAGA,59.16,59.96,22,22,50.00,50.00,chr17:41201097-41201118,chr17:41201282-41201304,207,46.38,chr17:41201097-41201304,1.1681,chr17,41201118,41201282,Target=chr17:41201119-41201282
T1P45,chr17:41209063-41209157,GTGGTGGGGTGAGATTTTTGTC,GTCTGCTCCACTTCCATTGAAG,60.22,59.77,22,22,50.00,50.00,chr17:41209024-41209045,chr17:41209201-41209223,199,46.23,chr17:41209024-41209223,0.8061,chr17,41209045,41209201,Target=chr17:41209046-41209201
T1P46,chr17:41215885-41215973,TGTTAAAGGGAGGAGGGGAGA,AGTGGTGTTTTCAGCCTCTGA,60.10,60.03,21,21,52.38,47.62,chr17:41215852-41215872,chr17:41216108-41216129,277,38.99,chr17:41215852-41216129,2.0357,chr17,41215872,41216108,Target=chr17:41215873-41216108
T1P47,chr17:41222939-41223099,CTTTCCAGAATGTTGTTAAGTCTTAGTC,GCAACATACCATCTTCAACCTCTG,59.31,60.43,28,24,35.71,45.83,chr17:41222887-41222914,chr17:41223135-41223159,272,44.12,chr17:41222887-41223159,4.4910,chr17,41222914,41223135,
T1P48,chr17:41226342-41226543,GTGTTTGTTCCAATACAGCAGATG,CCTTTCACAATTGGTGGCGATG,59.36,61.19,24,22,41.67,50.00,chr17:41226314-41226337,chr17:41226565-41226587,273,43.96,chr17:41226314-41226587,2.5531,chr17,41226337,41226565,Target=chr17:41226338-41226565
T1P49,chr17:41231345-41231421,GGGAAGGCTCAGATACAAACAC,GTACCCGTTCCCTTGATGTCTA,59.50,59.76,22,22,50.00,50.00,chr17:41231235-41231256,chr17:41231436-41231458,223,41.70,chr17:41231235-41231458,1.2842,chr17,41231256,41231436,Target=chr17:41231257-41231436
T1P50,chr17:41242955-41243054,AATGCAAAGGACACCACACAC,GTTGCAGCGTTTATAGTCTGCT,60.10,59.84,21,22,47.62,45.45,chr17:41242908-41242928,chr17:41243082-41243104,196,44.90,chr17:41242908-41243104,1.2948,chr17,41242928,41243082,Target=chr17:41242929-41243082
T1P51,chr17:41243623-41243783,TCCTTGTCACTCAGACCAACTC,GTTGCTACCGAGTGTCTGTCT,60.16,60.24,22,21,50.00,52.38,chr17:41243531-41243552,chr17:41243788-41243809,278,41.37,chr17:41243531-41243809,1.0001,chr17,41243552,41243788,
T1P52,chr17:41243934-41244094,GGGAAGCTCTTCATCCTCACTAG,GCATCTCAGGTTTGTTCTGAGAC,60.46,60.08,23,23,52.17,47.83,chr17:41243875-41243897,chr17:41244101-41244124,249,43.78,chr17:41243875-41244124,1.2460,chr17,41243897,41244101,
T1P53,chr17:41244246-41244406,TGGAGAGAAATCTGTATTAACAGTCTG,GAAGCCAGCTCAAGCAATATTAATG,59.29,59.61,27,25,37.04,40.00,chr17:41244169-41244195,chr17:41244411-41244436,267,37.08,chr17:41244169-41244436,6.2529,chr17,41244195,41244411,
T1P54,chr17:41244581-41244741,CTTTCAGGTGACATTGAATGTTCCT,CAGACAGTTAATATCACTGCAGGC,60.30,60.25,25,24,40.00,45.83,chr17:41244512-41244536,chr17:41244763-41244787,275,37.82,chr17:41244512-41244787,3.8465,chr17,41244536,41244763,
T1P55,chr17:41244910-41245070,AGTGGGCAGAGAATGTTGCA,TGTGAGTCAGTGTGCAGCAT,60.41,60.40,20,20,50.00,50.00,chr17:41244882-41244901,chr17:41245125-41245145,263,41.06,chr17:41244882-41245145,1.2952,chr17,41244901,41245125,
T1P56,chr17:41245287-41245447,GATCTTTCAGTTTGCAAAACCCTT,GGTAAAGAACCTGCAACTGGAG,59.16,59.70,24,22,37.50,50.00,chr17:41245259-41245282,chr17:41245515-41245537,278,38.13,chr17:41245259-41245537,4.1947,chr17,41245282,41245515,
T1P57,chr17:41245671-41245831,CAATTAGGTGGGCTTAGATTTCTACTG,AGTGGTCATGAGAATAAAACAAAAGGT,60.32,60.27,27,27,40.74,33.33,chr17:41245640-41245666,chr17:41245870-41245897,257,36.58,chr17:41245640-41245897,6.0717,chr17,41245666,41245870,
T1P58,chr17:41246036-41246196,TCAGGATGAAGGCCTGATGTAG,GACTTACTGGCCAGTGATCCT,59.83,59.69,22,21,50.00,52.38,chr17:41246003-41246024,chr17:41246239-41246260,257,39.30,chr17:41246003-41246260,2.2343,chr17,41246024,41246239,
T1P59,chr17:41246436-41246596,CCATCATGTGAGTCATCAGAACC,GCAAACAGCCTGGCTTAGC,59.65,60.28,23,19,47.83,57.89,chr17:41246345-41246367,chr17:41246606-41246625,280,42.86,chr17:41246345-41246625,2.8735,chr17,41246367,41246606,
T1P60,chr17:41246757-41246882,GCTGTAATGAGCTGGCATGAG,CAGTTGGTTGATTTCCACCTCC,59.90,59.96,21,22,52.38,50.00,chr17:41246694-41246714,chr17:41246951-41246973,279,38.71,chr17:41246694-41246973,1.8746,chr17,41246714,41246951,
T1P61,chr17:41249255-41249311,CCAGCTTCATAGACAAAGGTTCTC,CCATGCCTTTAACCACTTCTCTG,59.89,60.08,24,23,45.83,47.83,chr17:41249226-41249249,chr17:41249460-41249483,257,33.07,chr17:41249226-41249483,4.7006,chr17,41249249,41249460,Target=chr17:41249250-41249460
T1P62,chr17:41256879-41256978,TGAGTTTTCATGGACAGCACTTG,CACTTGCTGAGTGTGTTTCTCAA,60.21,60.15,23,23,43.48,43.48,chr17:41256831-41256853,chr17:41256988-41257011,180,37.78,chr17:41256831-41257011,4.3246,chr17,41256853,41256988,Target=chr17:41256854-41256988
T1P63,chr17:41258467-41258555,TCCTACTGTGGTTGCTTCCAAC,ATGGCTCTTAAGGGCAGTTGTG,61.02,61.42,22,22,50.00,50.00,chr17:41258425-41258446,chr17:41258641-41258663,238,33.61,chr17:41258425-41258663,4.3126,chr17,41258446,41258641,Target=chr17:41258447-41258641
T1P64,chr17:41276028-41276137,GGAATCCCAAATTAATACACTCTTGTG,CGTTGTCATTAGTTCTTTGGTTTGTAT,59.34,59.46,27,27,37.04,33.33,chr17:41275997-41276023,chr17:41276247-41276274,277,28.52,chr17:41275997-41276274,9.6448,chr17,41276023,41276247,Target=chr17:41276024-41276247

Tube 2: 64x; Tm: 60.05 (58.05-61.79); GC(%): 44.03 (22.58-60.00); PrimerSize: 23.91 (19-32); AmpliconGC(%): 37.78 (26.79-58.23); AmpliconSize: 264.20 (160-280); Penalty: 4.99 (0.99-20.21)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T2P1,chr13:32890553-32890669,CCAGCGCTTCTGAGTTTTACC,ACACTGTGACGTACTGGGTTT,60.04,60.04,21,21,52.38,47.62,chr13:32890477-32890497,chr13:32890732-32890753,276,36.23,chr13:32890477-32890753,2.9468,chr13,32890497,32890732,Target=chr13:32890498-32890732
T2P2,chr13:32893402-32893467,TCAGCTGGCTTCAACTCCAA,agaggccagagagactgatttg,60.04,60.03,20,22,50.00,50.00,chr13:32893361-32893380,chr13:32893563-32893585,224,39.29,chr13:32893361-32893585,2.3908,chr13,32893380,32893563,
T2P3,chr13:32900232-32900292,TTCCAACAATTTATATGAATGAGAATCTTC,GTGTATGAAACAAACTCCCACATACC,58.05,60.89,30,26,26.67,42.31,chr13:32900133-32900162,chr13:32900379-32900405,272,27.94,chr13:32900133-32900405,11.9661,chr13,32900162,32900379,Target=chr13:32900163-32900379
T2P4,chr13:32900630-32900755,CTGCCTCATACAGGCAATTCAG,CAACCTCATCTGCTCTTTCTTGT,59.90,59.45,22,23,50.00,43.48,chr13:32900519-32900540,chr13:32900773-32900796,277,37.18,chr13:32900519-32900796,3.1995,chr13,32900540,32900773,Target=chr13:32900541-32900773
T2P5,chr13:32905050-32905172,gggactactACTATATGTGCATTGAG,cagagcaagactccacctcaaa,59.22,60.75,26,22,42.31,50.00,chr13:32904990-32905015,chr13:32905228-32905250,260,32.69,chr13:32904990-32905250,6.6627,chr13,32905015,32905228,Target=chr13:32905016-32905228
T2P6,chr13:32906564-32906724,TGCCAAATGTCCTAGAAGATGAAG,CTTCCACTCTCAAAGGGCTTCT,59.34,60.49,24,22,41.67,50.00,chr13:32906474-32906497,chr13:32906732-32906754,280,31.79,chr13:32906474-32906754,5.9475,chr13,32906497,32906732,
T2P7,chr13:32906926-32907086,CCCCTATTGCATATTTCTTCATGTGA,TACCCTGAAATGAAGAAGCCACTG,60.05,61.35,26,24,38.46,45.83,chr13:32906848-32906873,chr13:32907092-32907116,268,37.31,chr13:32906848-32907116,5.0099,chr13,32906873,32907092,
T2P8,chr13:32907238-32907398,CTGAAGCCTCTGAAAGTGGACT,GCTTCAAACTGGGCTGAACAG,60.49,60.51,22,21,50.00,52.38,chr13:32907212-32907233,chr13:32907459-32907480,268,35.82,chr13:32907212-32907480,3.1567,chr13,32907233,32907459,
T2P9,chr13:32910396-32910556,TGTGATTGATGGTACTTTAATTTTGTCAC,GCATGACAGAGAATCAGCTTCTG,59.98,60.21,29,23,31.03,47.83,chr13:32910353-32910381,chr13:32910602-32910625,272,32.35,chr13:32910353-32910625,8.1612,chr13,32910381,32910602,
T2P10,chr13:32910779-32910939,AGTGATACTGACTTTCAATCCCAGA,CTCTCATGTATTTTTCAGGTGGCA,60.06,59.83,25,24,40.00,41.67,chr13:32910736-32910760,chr13:32910992-32911016,280,33.21,chr13:32910736-32911016,5.9158,chr13,32910760,32910992,
T2P11,chr13:32911150-32911310,CTGTCAATCCAGACTCTGAAGAAC,TCTCCTCTGCAAGAACATAAACCA,59.59,60.50,24,24,45.83,41.67,chr13:32911112-32911135,chr13:32911331-32911355,243,35.39,chr13:32911112-32911355,5.0949,chr13,32911135,32911331,
T2P12,chr13:32911488-32911648,TGAACAAATGGGCAGGACTCT,ACTGAGGCTTGCTCAGTTTCT,60.10,60.10,21,21,47.62,47.62,chr13:32911460-32911480,chr13:32911661-32911682,222,35.14,chr13:32911460-32911682,3.8264,chr13,32911480,32911661,
T2P13,chr13:32911842-32912002,ACACCTAGCCAAAAGGCAGA,TTGCTTGCTGCTGTCTACCT,59.75,60.12,20,20,50.00,50.00,chr13:32911801-32911820,chr13:32912015-32912035,234,38.89,chr13:32911801-32912035,2.4374,chr13,32911820,32912015,
T2P14,chr13:32912175-32912335,GGGGCTTTTATTCTGCTCATGG,CTTCAACAAAAGTGCCAGTAGTCA,60.16,60.19,22,24,50.00,41.67,chr13:32912141-32912162,chr13:32912390-32912414,273,31.14,chr13:32912141-32912414,5.6915,chr13,32912162,32912390,
T2P15,chr13:32912537-32912697,CTGCTGCCAGTAGAAATTCTCA,TTGCTCCGTTTTAGTAGCAGTT,58.91,58.77,22,22,45.45,40.91,chr13:32912465-32912486,chr13:32912718-32912740,275,34.18,chr13:32912465-32912740,5.8574,chr13,32912486,32912718,
T2P16,chr13:32912905-32913065,TGATCAGAAACCAGAAGAATTGCAT,CACTTTGTCCAAAGATTCCTTTGC,59.83,59.77,25,24,36.00,41.67,chr13:32912841-32912865,chr13:32913094-32913118,277,35.74,chr13:32912841-32913118,5.4659,chr13,32912865,32913094,
T2P17,chr13:32913242-32913402,CAGAGAGGCCTGTAAAGACCTT,CAAGTTGCAGGACTTTTTGCTG,59.96,59.64,22,22,50.00,45.45,chr13:32913198-32913219,chr13:32913431-32913453,255,34.90,chr13:32913198-32913453,3.6484,chr13,32913219,32913431,
T2P18,chr13:32913580-32913740,CTTCTGTGAGTCAGACTTCATTACTTG,TCCTGAATCATTATATACCTCATCAGAATG,60.42,59.68,27,30,40.74,33.33,chr13:32913527-32913553,chr13:32913745-32913775,248,31.45,chr13:32913527-32913775,8.9240,chr13,32913553,32913745,
T2P19,chr13:32913908-32914068,CCAATGTAAAAGATGCAAATGCATACC,CTGAAACTGTCTGTAAATATGTCTTTCAC,60.80,59.84,27,29,37.04,34.48,chr13:32913866-32913892,chr13:32914075-32914104,238,34.87,chr13:32913866-32914104,7.9116,chr13,32913892,32914075,
T2P20,chr13:32914238-32914398,CGAGGCATTGGATGATTCAGAG,CAGATTTTCCACTTGCTGTGCT,59.71,60.22,22,22,50.00,45.45,chr13:32914173-32914194,chr13:32914426-32914448,275,34.91,chr13:32914173-32914448,3.5062,chr13,32914194,32914426,
T2P21,chr13:32914628-32914788,TCAGACCAGCTCACAAGAGAAG,GGTTTCTCTTATCAACACGAGGAAG,60.22,60.18,22,25,50.00,44.00,chr13:32914555-32914576,chr13:32914807-32914832,277,35.38,chr13:32914555-32914832,4.4091,chr13,32914576,32914807,
T2P22,chr13:32914982-32915142,TCCATATCTCTCTCAATTTCAACAAGAC,CAGTTTAGAATCTGTCAGTTCATCATCT,60.04,60.04,28,28,35.71,35.71,chr13:32914947-32914974,chr13:32915199-32915227,280,33.21,chr13:32914947-32915227,7.2312,chr13,32914974,32915199,
T2P23,chr13:32918689-32918795,TGTTTAACATTTAAAGAGTCAATACTTTAGCT,acctgaggtcagaaTATTATATACCATACC,59.64,60.52,32,30,25.00,36.67,chr13:32918580-32918611,chr13:32918830-32918860,280,26.79,chr13:32918580-32918860,11.9554,chr13,32918611,32918830,Target=chr13:32918612-32918830
T2P24,chr13:32928992-32929152,GGTCTGCAACAAAGGCATATTCC,GGTTGGTCTGCCTGTAGTAATCA,60.96,60.59,23,23,47.83,47.83,chr13:32928919-32928941,chr13:32929176-32929199,280,35.00,chr13:32928919-32929199,3.9185,chr13,32928941,32929176,
T2P25,chr13:32929358-32929430,CATTGATGGACATGGCTCTGATG,AAGGGCTTTAAAATTACCACCACC,60.27,60.26,23,24,47.83,41.67,chr13:32929297-32929319,chr13:32929513-32929537,240,34.17,chr13:32929297-32929537,4.7987,chr13,32929319,32929513,
T2P26,chr13:32931873-32932041,TTGTGTGATACATGTTTACTTTAAATTGTTT,CACACAATCTTTTTGCATAGAGTACCTA,58.92,60.46,31,28,22.58,35.71,chr13:32931817-32931847,chr13:32932063-32932091,274,33.94,chr13:32931817-32932091,20.2057,chr13,32931847,32932063,
T2P27,chr13:32936654-32936835,GTTGAATTCAGTATCATCCTATGTGGT,ACCATACTGCCGTATATGATTACGTA,59.83,60.17,27,26,37.04,38.46,chr13:32936599-32936625,chr13:32936850-32936876,277,36.82,chr13:32936599-32936876,5.6653,chr13,32936625,32936850,Target=chr13:32936626-32936850
T2P28,chr13:32937508-32937668,CCCAAAAAGTGGCCATTATTGAAC,TGACTGATTTTTACCAAGAGTGCAA,59.83,59.95,24,25,41.67,36.00,chr13:32937476-32937499,chr13:32937680-32937705,229,43.23,chr13:32937476-32937705,3.3117,chr13,32937499,32937680,
T2P29,chr13:32944666-32944699,TCGCTTTTCAGTGATGGAGGA,tggtggctcagctacttgag,59.90,59.90,21,20,47.62,55.00,chr13:32944625-32944645,chr13:32944866-32944886,261,42.91,chr13:32944625-32944886,1.4301,chr13,32944645,32944866,
T2P30,chr13:32950801-32950933,CTTTGGGTGTTTTATGCTTGGTTC,GAGAGTCTAAAACAGCTTCTCACC,59.77,59.59,24,24,41.67,45.83,chr13:32950720-32950743,chr13:32950973-32950997,277,37.55,chr13:32950720-32950997,3.7890,chr13,32950743,32950973,Target=chr13:32950744-32950973
T2P31,chr13:32953632-32953657,GAAACAAGCTCAGATCCAGTTGG,CTCTTTGTGGGCATTAGTAGTGG,60.33,59.58,23,23,47.83,47.83,chr13:32953515-32953537,chr13:32953770-32953793,278,32.73,chr13:32953515-32953793,4.7691,chr13,32953537,32953770,
T2P32,chr13:32954138-32954287,CTTGTTAGTTTATGGAATCTCCATATGTTG,GGTAGCTCCAACTAATCATAAGAGATTTTA,59.78,59.98,30,30,33.33,33.33,chr13:32954090-32954119,chr13:32954338-32954368,278,32.37,chr13:32954090-32954368,8.5954,chr13,32954119,32954338,Target=chr13:32954120-32954338
T2P33,chr13:32969010-32969075,GCCCCTTTCGTCTATTTGTCAG,CCAAAATGTGTGGTGATGCTGA,59.83,60.22,22,22,50.00,45.45,chr13:32968830-32968851,chr13:32969083-32969105,275,38.91,chr13:32968830-32969105,1.9885,chr13,32968851,32969083,
T2P34,chr13:32972293-32972453,TGTTACTACATAATTATGATAGGCTACGT,GTACAAATGGGACTAACAGGTGGAG,58.91,61.63,29,25,31.03,48.00,chr13:32972252-32972280,chr13:32972488-32972513,261,37.93,chr13:32972252-32972513,7.0783,chr13,32972280,32972488,
T2P35,chr13:32972685-32972845,CTGAATTCTCCTCAGATGACTCCAT,TGCTTATTTTTCTCACATTCTTCCGT,60.47,60.34,25,26,44.00,34.62,chr13:32972598-32972622,chr13:32972850-32972876,278,38.13,chr13:32972598-32972876,4.8651,chr13,32972622,32972850,
T2P36,chr13:32973031-32973191,CGCTTAACCTTTCCAGTTTATAAGACT,actcctcccacctcagctt,60.21,60.36,27,19,37.04,57.89,chr13:32972937-32972963,chr13:32973198-32973217,280,36.79,chr13:32972937-32973217,7.5583,chr13,32972963,32973198,
T2P37,chr13:32973379-32973539,TTTGATCACTACAAGTATTATTTTACAAGTGA,GATGAAGAATTGCTCAAAAGGAAACAC,59.27,60.64,32,27,25.00,37.04,chr13:32973319-32973350,chr13:32973566-32973593,274,31.02,chr13:32973319-32973593,11.1880,chr13,32973350,32973566,
T2P38,chr13:32973745-32973814,GAACCCTCAGTGTAACTCTAATTCC,TGTGGTAAGTTTAAAAGGGCATAGG,59.42,59.83,25,25,44.00,40.00,chr13:32973622-32973646,chr13:32973848-32973873,251,31.87,chr13:32973622-32973873,6.6577,chr13,32973646,32973848,
T2P39,chr17:41196517-41196677,CACTTTTTCTTCCTTCAGCAAGC,GAACCGGTTTCCAAAGACAGTC,59.46,60.22,23,22,43.48,50.00,chr17:41196476-41196498,chr17:41196708-41196730,254,35.04,chr17:41196476-41196730,4.1926,chr17,41196498,41196708,
T2P40,chr17:41196869-41197029,ACTTTTAGAAATCTCTTCTAGtttcattttcc,actggaggtcaggagttcgaaa,59.88,61.56,32,22,28.12,50.00,chr17:41196789-41196820,chr17:41197036-41197058,269,47.21,chr17:41196789-41197058,8.3589,chr17,41196820,41197036,
T2P41,chr17:41197183-41197343,CAGAATTTCCTCCCCAATGTTCC,CCAATTGAGCAAGATGCTGATTC,60.08,59.15,23,23,47.83,43.48,chr17:41197153-41197175,chr17:41197403-41197426,273,44.32,chr17:41197153-41197426,2.0866,chr17,41197175,41197403,
T2P42,chr17:41197571-41197731,CTTTAAGGGACCCTTGCATAGC,TTGGACAGTGTAGCACTCTACC,59.57,59.96,22,22,50.00,50.00,chr17:41197520-41197541,chr17:41197750-41197772,252,52.38,chr17:41197520-41197772,3.4998,chr17,41197541,41197750,
T2P43,chr17:41199654-41199725,ACCCCATATAGCACAGGTACATG,gaagtgacagttccagtagtcct,60.21,59.96,23,23,47.83,47.83,chr17:41199624-41199646,chr17:41199761-41199784,160,51.25,chr17:41199624-41199784,2.3741,chr17,41199646,41199761,Target=chr17:41199647-41199761
T2P44,chr17:41203074-41203139,CCCATCGTGGGATCTTGCTT,CATCAGGTGGTGAACAGAAGAA,60.62,58.77,20,22,55.00,45.45,chr17:41202995-41203014,chr17:41203251-41203273,278,44.24,chr17:41202995-41203273,2.6451,chr17,41203014,41203251,Target=chr17:41203015-41203251
T2P45,chr17:41215344-41215395,GGAAAGTGGTGCATTGATGGAA,GCTTTAGAGGGAAGGACCTCTC,59.96,60.09,22,22,45.45,54.55,chr17:41215256-41215277,chr17:41215498-41215520,264,39.77,chr17:41215256-41215520,2.0110,chr17,41215277,41215498,Target=chr17:41215278-41215498
T2P46,chr17:41219619-41219717,ggtctcgatctcctaatctcgt,AGCTGTGTGCTAGAGGTAACTC,59.11,60.03,22,22,50.00,50.00,chr17:41219486-41219507,chr17:41219742-41219764,278,43.88,chr17:41219486-41219764,0.9938,chr17,41219507,41219742,Target=chr17:41219508-41219742
T2P47,chr17:41223125-41223260,ACCCAGCAGTATCAGTAGTATGAG,ACAGAGACCAGAACTTTGTAATTCA,59.71,58.94,24,25,45.83,36.00,chr17:41223057-41223080,chr17:41223298-41223323,266,45.86,chr17:41223057-41223323,3.9029,chr17,41223080,41223298,
T2P48,chr17:41228499-41228636,AAAGATGTCAGATACCACAGCATC,TCACTATCAGAACAAAGCAGTAAAGT,59.41,59.32,24,26,41.67,34.62,chr17:41228460-41228483,chr17:41228658-41228684,224,36.61,chr17:41228460-41228684,6.8202,chr17,41228483,41228658,Target=chr17:41228484-41228658
T2P49,chr17:41234415-41234597,GATAAAGGGGAAGGAAAGAATTTTGC,CTCAAAGTATTTCATTTTCTTGGTGCC,59.72,60.43,26,27,38.46,37.04,chr17:41234361-41234386,chr17:41234609-41234636,275,42.55,chr17:41234361-41234636,4.4315,chr17,41234386,41234609,Target=chr17:41234387-41234609
T2P50,chr17:41243446-41243606,CTGGGGCAAACACAAAAACCT,CACAGTGCAGTGAATTGGAAGA,60.31,59.64,21,22,47.62,45.45,chr17:41243419-41243439,chr17:41243633-41243655,236,42.80,chr17:41243419-41243655,1.7561,chr17,41243439,41243633,
T2P51,chr17:41243778-41243938,GATGTTCCTGAGATGCCTTTGC,CCATACACATTTGGCTCAGGGT,60.42,61.16,22,22,50.00,50.00,chr17:41243697-41243718,chr17:41243944-41243966,269,41.64,chr17:41243697-41243966,1.0580,chr17,41243718,41243944,
T2P52,chr17:41244091-41244251,CTGGACGCTTTTGCTAAAAACAG,GCAACCTGAGGTCTATAAACAAAGT,59.78,59.83,23,25,43.48,40.00,chr17:41244001-41244023,chr17:41244256-41244281,280,36.79,chr17:41244001-41244281,4.6640,chr17,41244023,41244256,
T2P53,chr17:41244401-41244561,TGCTTGAATGTTTTCATCACTGGA,TCGTATACCACCACTTTTTCCCA,59.95,60.21,24,23,37.50,43.48,chr17:41244337-41244360,chr17:41244591-41244614,277,35.02,chr17:41244337-41244614,5.1734,chr17,41244360,41244591,
T2P54,chr17:41244753-41244913,TTCGTTGCCTCTGAACTGAGA,TGCTCCGTTTTCAAATCCAGG,59.83,59.62,21,21,47.62,47.62,chr17:41244661-41244681,chr17:41244920-41244941,280,40.36,chr17:41244661-41244941,1.8505,chr17,41244681,41244920,
T2P55,chr17:41245115-41245275,GTCCCAATGGATACTTAAAGCCTTC,TGCTGAAGACCCCAAAGATCTC,60.24,60.56,25,22,44.00,50.00,chr17:41245044-41245068,chr17:41245297-41245319,275,40.73,chr17:41245044-41245319,2.6767,chr17,41245068,41245297,
T2P56,chr17:41245505-41245665,GAACCAGGTGCATTTGTTAACTTC,CTTCTACCAGGCATATTCATGCG,59.54,60.08,24,23,41.67,47.83,chr17:41245424-41245447,chr17:41245681-41245704,280,40.71,chr17:41245424-41245704,2.4900,chr17,41245447,41245681,
T2P57,chr17:41245860-41246020,GGTTCAGCTTTCGTTTTGAAAGC,CAAGAGCGTCCCCTCACAAATA,60.27,60.88,23,22,43.48,50.00,chr17:41245790-41245812,chr17:41246046-41246068,278,37.41,chr17:41245790-41246068,3.2710,chr17,41245812,41246046,
T2P58,chr17:41246229-41246389,ACTGATTTGGAGTGAACTCTTTCAC,CATGCTCAGAGAATCCTAGAGATACT,60.01,59.89,25,26,40.00,42.31,chr17:41246192-41246216,chr17:41246446-41246472,280,38.57,chr17:41246192-41246472,4.0819,chr17,41246216,41246446,
T2P59,chr17:41246596-41246756,GGGATCAGCATTCAGATCTACCT,AGCTGAGAGGCATCCAGAAAAG,59.95,60.89,23,22,47.83,50.00,chr17:41246509-41246531,chr17:41246767-41246789,280,42.86,chr17:41246509-41246789,1.1405,chr17,41246531,41246767,
T2P60,chr17:41247857-41247944,CCACTCTCTTTTCAGTGCCTGT,AGGAGAGAGCAGCTTTCACTAAC,61.02,60.59,22,23,50.00,47.83,chr17:41247816-41247837,chr17:41248059-41248082,266,39.10,chr17:41247816-41248082,2.3580,chr17,41247837,41248059,Target=chr17:41247838-41248059
T2P61,chr17:41251786-41251902,TTCACTTCCCAAAGCTGCCTA,GGAAAAGCACAGAACTGGCC,60.10,60.19,21,20,47.62,55.00,chr17:41251674-41251694,chr17:41251933-41251953,279,39.43,chr17:41251674-41251953,2.2476,chr17,41251694,41251933,Target=chr17:41251695-41251933
T2P62,chr17:41256133-41256283,ttctagcctgggccacagag,CTCTAACTGCAAACATAATGTTTTCCCT,61.79,61.09,20,28,60.00,35.71,chr17:41256040-41256059,chr17:41256290-41256318,278,34.89,chr17:41256040-41256318,9.2741,chr17,41256059,41256290,Target=chr17:41256060-41256290
T2P63,chr17:41267737-41267801,GGTGTTTCCTGGGTTATGAAGGA,cgaaCTTGAGGCCTTATGTTGAC,60.79,60.39,23,23,47.83,47.83,chr17:41267672-41267694,chr17:41267903-41267926,254,37.40,chr17:41267672-41267926,3.1342,chr17,41267694,41267903,Target=chr17:41267695-41267903
T2P64,chr17:41277282-41277386,CATCTGTCAGCTTCGGAAATCC,GGAAAAGCGCGGGAATTACAG,59.90,60.71,22,21,50.00,52.38,chr17:41277199-41277220,chr17:41277415-41277436,237,58.23,chr17:41277199-41277436,5.9806,chr17,41277220,41277415,Target=chr17:41277221-41277415

Designed by MP-Ref