Primer Design Reports (2025-07-18 14:25:41)
[0-based position]

Tube 1: 49x; Tm: 59.91 (58.45-61.29); GC(%): 47.75 (33.33-63.16); PrimerSize: 22.47 (18-27); AmpliconGC(%): 44.87 (36.33-54.94); AmpliconSize: 245.80 (161-260); Penalty: 2.86 (0.73-13.94)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T1P1,chrM:30-160,GATCACAGGTCTATCACCCTATTAAC,ACACACTTTAGTAAGTATGTTCGCC,59.16,59.89,26,25,42.31,40.00,chrM:0-25,chrM:183-208,208,47.60,chrM:0-208,13.9380,chrM,25,183,
T1P2,chrM:328-488,AGCCACTTTCCACACAGACA,GTGTGCTGGGTAGGATGGG,59.97,60.28,20,19,50.00,63.16,chrM:258-277,chrM:499-518,260,46.54,chrM:258-518,3.4772,chrM,277,499,
T1P3,chrM:646-806,TGAAAATGTTTAGACGGGCTCAC,TTGCTAAAGGTTAATCACTGCTGT,60.02,59.46,23,24,43.48,37.50,chrM:609-631,chrM:813-837,228,45.61,chrM:609-837,3.0935,chrM,631,813,
T1P4,chrM:990-1150,ACCCCCTCCCCAATAAAGCTA,ACAGGCTCCTCTAGAGGGATAT,60.82,59.54,21,22,52.38,50.00,chrM:954-974,chrM:1185-1207,253,43.87,chrM:954-1207,1.3390,chrM,974,1185,
T1P5,chrM:1335-1495,AGTACCCACGTAAAGACGTTAGG,TCTCCTCTATATAAATGCGTAGGGG,60.33,59.65,23,25,47.83,44.00,chrM:1305-1327,chrM:1530-1555,250,45.60,chrM:1305-1555,2.6984,chrM,1327,1530,
T1P6,chrM:1681-1841,AACTTGACCGCTCTGAGCTA,GCTTTGGCTCTCCTTGCAAA,59.25,59.83,20,20,50.00,50.00,chrM:1654-1673,chrM:1875-1895,241,40.25,chrM:1654-1895,2.3585,chrM,1673,1875,
T1P7,chrM:2048-2208,CTGGTGATAGCTGGTTGTCCA,TTGGGTGTGAGGAGTTCAGTT,60.24,59.68,21,21,52.38,47.62,chrM:2005-2025,chrM:2244-2265,260,39.23,chrM:2005-2265,2.5602,chrM,2025,2244,
T1P8,chrM:2422-2582,AACAGCCCAATATCTACAATCAACC,aggtccctatttaaggaacaagtga,59.95,60.00,25,25,40.00,40.00,chrM:2372-2396,chrM:2601-2626,254,44.88,chrM:2372-2626,3.4311,chrM,2396,2601,
T1P9,chrM:2759-2919,caagacgagaagaccctatgga,AGGATTGCGCTGTTATCCCTAG,59.50,60.49,22,22,50.00,50.00,chrM:2712-2733,chrM:2928-2950,238,44.12,chrM:2712-2950,0.9618,chrM,2733,2928,
T1P10,chrM:3099-3259,GAGTTCAGACCGGAGTAATCCA,GTTGGCCATGGGTAtgttgtt,59.76,59.62,22,21,50.00,47.62,chrM:3067-3088,chrM:3300-3321,254,43.31,chrM:3067-3321,1.0494,chrM,3088,3300,
T1P11,chrM:3446-3606,CAAAGGCCCCAACGTTGTAG,CTAGGCTAGAGGTGGCTAGAAT,59.90,58.75,20,22,55.00,50.00,chrM:3407-3426,chrM:3618-3640,233,54.94,chrM:3407-3640,5.1490,chrM,3426,3618,
T1P12,chrM:3766-3926,AGTCACCCTAGCCATCATTCTAC,GCTATGAAGAATAGGGCGAAGG,59.89,59.18,23,22,47.83,50.00,chrM:3734-3756,chrM:3961-3983,249,48.59,chrM:3734-3983,2.8559,chrM,3756,3961,
T1P13,chrM:4141-4301,CCTCCCTGTTCTTATGAATTCGAAC,ggatgggttcgattctcatagtcc,60.24,61.10,25,24,44.00,50.00,chrM:4105-4129,chrM:4331-4355,250,40.80,chrM:4105-4355,2.5948,chrM,4129,4331,
T1P14,chrM:4476-4636,AATAAGCTATCGGGCCCATACC,GTTGCTTGCGTGAGGAAATACT,60.29,60.03,22,22,50.00,45.45,chrM:4415-4436,chrM:4641-4663,248,44.76,chrM:4415-4663,1.3334,chrM,4436,4641,
T1P15,chrM:4787-4947,GACAATGAACCATAACCAATACTACCA,ACCTCAACTGCCTGCTATGAT,59.99,59.68,27,21,37.04,47.62,chrM:4713-4739,chrM:4952-4973,260,42.69,chrM:4713-4973,4.3815,chrM,4739,4952,
T1P16,chrM:5137-5297,TCCTAACTACTACCGCATTCCTAC,GGTAGAAGTAGAGGTTAAGGAGGG,59.71,59.64,24,24,45.83,50.00,chrM:5097-5120,chrM:5325-5349,252,48.41,chrM:5097-5349,3.0003,chrM,5120,5325,
T1P17,chrM:5510-5670,GCTACTCCTACCTATCTCCCCTT,CAGTTGATTAGGGTGCTTAGCTG,60.47,59.90,23,23,52.17,47.83,chrM:5470-5492,chrM:5695-5718,248,39.11,chrM:5470-5718,2.4239,chrM,5492,5695,
T1P18,chrM:5860-6020,GCTGGTAAAAAGAGGCCTAACC,TCGTTACCTAGAAGGTTGCCTG,59.76,60.29,22,22,50.00,50.00,chrM:5823-5844,chrM:6033-6055,232,49.57,chrM:5823-6055,2.4692,chrM,5844,6033,
T1P19,chrM:6196-6356,CTAATAATCGGTGCCCCCGAT,ATGGCCCCTAAGATAGAGGAGA,60.24,59.81,21,22,52.38,50.00,chrM:6155-6175,chrM:6366-6388,233,53.22,chrM:6155-6388,4.0040,chrM,6175,6366,
T1P20,chrM:6517-6677,TTCTCCTATCTCTCCCAGTCCTAG,AGCTCAGACCATACCTATGTATCC,60.19,59.52,24,24,50.00,45.83,chrM:6486-6509,chrM:6707-6731,245,45.31,chrM:6486-6731,1.9216,chrM,6509,6707,
T1P21,chrM:6852-7012,GAGCATATTTCACCTCCGCTAC,GACATAGTGGAAGTGGGCTACA,59.45,60.03,22,22,50.00,50.00,chrM:6807-6828,chrM:7021-7043,236,47.88,chrM:6807-7043,1.7125,chrM,6828,7021,
T1P22,chrM:7173-7333,CCTACGCCAAAATCCATTTCACT,CACTCCAGGTTTATGGAGGGTT,60.08,60.23,23,22,43.48,50.00,chrM:7125-7147,chrM:7362-7384,259,42.47,chrM:7125-7384,1.3677,chrM,7147,7362,
T1P23,chrM:7487-7647,GACAaaaaaggaaggaatcgaacc,ACAGGACTAGGAAGCAGATAAGG,59.29,59.57,24,23,41.67,47.83,chrM:7443-7466,chrM:7680-7703,260,40.00,chrM:7443-7703,3.4863,chrM,7466,7680,
T1P24,chrM:7828-7988,GCCATCATCCTAGTCCTCATCG,ATCGGGAGTACTACTCGATTGTC,60.61,59.71,22,23,54.55,47.83,chrM:7792-7813,chrM:7999-8022,230,50.43,chrM:7792-8022,3.6358,chrM,7813,7999,
T1P25,chrM:8152-8312,CAAACCACTTTCACCGCTACAC,CACTGTAAAGAGGTGTTGGTTCTC,60.80,60.01,22,24,50.00,45.83,chrM:8125-8146,chrM:8339-8363,238,44.12,chrM:8125-8363,1.6989,chrM,8146,8339,
T1P26,chrM:8500-8660,CCTACCTCCCTCACCAAAGC,TCGTCCTTTAGTGTTGTGTATGGT,60.26,60.50,20,24,60.00,41.67,chrM:8466-8485,chrM:8700-8724,258,41.47,chrM:8466-8724,4.2737,chrM,8485,8700,
T1P27,chrM:8846-9006,GCCTCACTCATTTACACCAACC,GAGTAGGTGGCCTGCAGTAAT,60.03,60.03,22,21,50.00,52.38,chrM:8789-8810,chrM:9015-9036,247,48.18,chrM:8789-9036,2.5377,chrM,8810,9015,
T1P28,chrM:9161-9321,ATCGCTGTCGCCTTAATCCA,GTGTTACATCGCGCCATCAT,60.05,59.56,20,20,50.00,50.00,chrM:9135-9154,chrM:9373-9393,258,48.84,chrM:9135-9393,2.6478,chrM,9154,9373,
T1P29,chrM:9523-9683,TTCTGAGCCTTTTACCACTCCA,CTGAGGCTTGTAGGAGGGTAAA,59.82,59.69,22,22,45.45,50.00,chrM:9497-9518,chrM:9722-9744,247,47.37,chrM:9497-9744,2.0880,chrM,9518,9722,
T1P30,chrM:9881-10041,ACTATCTGCTTCATCCGCCAA,GTAGTAAGGCTAGGAGGGTGTT,60.03,59.15,21,22,47.62,50.00,chrM:9851-9871,chrM:10085-10107,256,36.33,chrM:9851-10107,3.4791,chrM,9871,10085,
T1P31,chrM:10234-10394,CCGCGTCCCTTTCTCCAT,TGAGTCGAAATCATTCGTTTTGTT,59.91,58.45,18,24,61.11,33.33,chrM:10198-10215,chrM:10418-10442,244,38.11,chrM:10198-10442,8.0542,chrM,10215,10418,
T1P32,chrM:10587-10747,CACCTCATATCCTCCCTACTATGC,GTTGGGACGATTAGTTTTAGCATTG,60.13,59.49,24,25,50.00,40.00,chrM:10541-10564,chrM:10757-10782,241,46.06,chrM:10541-10782,2.9183,chrM,10564,10757,
T1P33,chrM:10938-11098,CCTATTTAGCTGTTCCCCAACC,GATGATAGCCAAGGTGGGGATA,59.23,59.62,22,22,50.00,50.00,chrM:10902-10923,chrM:11136-11158,256,42.97,chrM:10902-11158,0.8653,chrM,10923,11136,
T1P34,chrM:11293-11453,TCACAACACCCTAGGCTCAC,TGAGAATGAGTGTGAGGCGT,59.83,59.54,20,20,55.00,50.00,chrM:11259-11278,chrM:11490-11510,251,43.82,chrM:11259-11510,1.4307,chrM,11278,11490,
T1P35,chrM:11636-11796,ACAGACCTAAAATCGCTCATTGC,TAGCGAGGCTTGCTAGAAGTC,60.15,60.11,23,21,43.48,52.38,chrM:11596-11618,chrM:11834-11855,259,46.33,chrM:11596-11855,3.1109,chrM,11618,11834,
T1P36,chrM:11985-12145,CTCAACATACTAGTCACAGCCCTA,CGTAAGCCTCTGTTGTCAGATTC,59.89,59.90,24,23,45.83,47.83,chrM:11950-11973,chrM:12169-12192,242,43.39,chrM:11950-12192,1.2101,chrM,11973,12169,
T1P37,chrM:12339-12499,GGCCCCAAAAATTTTGGTGCA,GTTGTTTGGGTTGTGGCTCAG,61.29,60.72,21,21,47.62,52.38,chrM:12299-12319,chrM:12536-12557,258,41.86,chrM:12299-12557,1.8008,chrM,12319,12536,
T1P38,chrM:12722-12882,ACTCAGACCCAAACATTAATCAGTTC,GTGTAGGATAAATCATGCTAAGGCG,60.11,60.41,26,25,38.46,44.00,chrM:12661-12686,chrM:12887-12912,251,42.63,chrM:12661-12912,2.8819,chrM,12686,12887,
T1P39,chrM:13053-13213,AATTAGGTCTCCACCCCTGACT,GTTGACTTGAAGTGGAGAAGGC,60.77,59.96,22,22,50.00,50.00,chrM:13012-13033,chrM:13242-13264,252,49.21,chrM:13012-13264,2.7197,chrM,13033,13242,
T1P40,chrM:13429-13589,CATCCACAACCTTAACAATGAACAAG,GAAGAATTATTCGAGTGCTATAGGCG,59.89,60.50,26,26,38.46,42.31,chrM:13373-13398,chrM:13595-13621,248,43.95,chrM:13373-13621,3.6597,chrM,13398,13595,
T1P41,chrM:13759-13919,AGCCTATTCGCAGGATTTCTCA,AAGGCCTAGATAGGGGATTGTG,60.09,59.55,22,22,45.45,50.00,chrM:13716-13737,chrM:13940-13962,246,45.12,chrM:13716-13962,1.5363,chrM,13737,13940,
T1P42,chrM:14089-14249,ACAGCACCAAATCTCCACCT,GGTTGATTCGGGAGGATCCTAT,59.75,59.62,20,22,50.00,50.00,chrM:14036-14055,chrM:14254-14276,240,42.08,chrM:14036-14276,1.6501,chrM,14055,14254,
T1P43,chrM:14425-14585,AATCCTACCTCCATCGCTAACC,TGTGGGGTTTTCTTCTAAGCCT,59.89,60.09,22,22,50.00,45.45,chrM:14368-14389,chrM:14604-14626,258,42.64,chrM:14368-14626,0.7312,chrM,14389,14604,
T1P44,chrM:14758-14918,CGACCAATGATATGAAAAACCATCG,GTGATGTGGGCGATTGATGAAA,59.61,60.09,25,22,40.00,45.45,chrM:14696-14720,chrM:14933-14955,259,47.10,chrM:14696-14955,4.2606,chrM,14720,14933,
T1P45,chrM:15112-15272,CAGAAACCTGAAACATCGGCAT,GCTAGGGCTGCAATAATGAAGG,60.03,59.96,22,22,45.45,50.00,chrM:15074-15095,chrM:15302-15324,250,46.80,chrM:15074-15324,1.8185,chrM,15095,15302,
T1P46,chrM:15485-15645,CCTCGGCTTACTTCTCTTCCTT,TTGGATATATGGAGGATGGGGATT,60.03,59.18,22,24,50.00,41.67,chrM:15432-15453,chrM:15654-15678,246,47.15,chrM:15432-15678,2.8018,chrM,15453,15654,
T1P47,chrM:15799-15959,GCAGACCTCCTCATTCTAACCT,TTGGGTGCTAATGGTGGAGTTA,59.76,59.89,22,22,50.00,45.45,chrM:15733-15754,chrM:15971-15993,260,39.62,chrM:15733-15993,1.8373,chrM,15754,15971,
T1P48,chrM:16117-16277,TCACCCATCAACAACCGCTA,ACTGTTAAGGGTGGGTAGGTTT,59.83,59.48,20,22,50.00,45.45,chrM:16067-16086,chrM:16282-16304,237,45.15,chrM:16067-16304,1.8319,chrM,16086,16282,
T1P49,chrM:16454-16509,CCATCCTCCGTGAAATCAATATCC,GATGTCTTATTTAAGGGGAACGTGT,59.83,59.65,24,25,45.83,40.00,chrM:16402-16425,chrM:16538-16563,161,49.07,chrM:16402-16563,3.1188,chrM,16425,16538,

Tube 2: 48x; Tm: 59.90 (58.43-61.31); GC(%): 47.32 (32.14-57.89); PrimerSize: 22.74 (19-28); AmpliconGC(%): 44.11 (34.50-52.40); AmpliconSize: 245.77 (206-260); Penalty: 2.96 (0.88-18.74)
ID,TemplateID,ForwardPrimer(Fp),ReversePrimer(Rp),FpTm,RpTm,FpSize,RpSize,FpGC(%),RpGC(%),FpPos,RpPos,AmpSize(bp),AmpGC,AmpPos,Penalty,Chr,Start,End,Note
T2P1,chrM:173-333,CACCCTATGTCGCAGTATCTGT,TTGGGGTTTGGCAGAGATGT,60.16,60.04,22,20,50.00,50.00,chrM:109-130,chrM:338-358,249,41.37,chrM:109-358,1.6851,chrM,130,338,
T2P2,chrM:489-649,CACATTATTTTCCCCTCCCACTC,AGAGCTAATAGAAAGGCTAGGACC,59.57,59.70,23,24,47.83,45.83,chrM:444-466,chrM:656-680,236,47.88,chrM:444-680,3.0025,chrM,466,656,
T2P3,chrM:803-963,CAGCTCAAAACGCTTAGCCTAG,TCGTAGTCTATTTTGTGTCAACTGG,60.16,59.60,22,25,50.00,40.00,chrM:774-795,chrM:1000-1025,251,45.42,chrM:774-1025,3.0108,chrM,795,1000,
T2P4,chrM:1175-1335,AACACTACGAGCCACAGCTT,TAGCCCATTTCTTGCCACCT,60.12,59.82,20,20,50.00,50.00,chrM:1138-1157,chrM:1345-1365,227,49.34,chrM:1138-1365,2.7067,chrM,1157,1345,
T2P5,chrM:1520-1680,CGTCACCCTCCTCAAGTATACTTC,GTTGTCTGGTAGTAAGGTGGAGT,60.73,59.95,24,23,50.00,47.83,chrM:1487-1510,chrM:1691-1714,227,44.05,chrM:1487-1714,1.7454,chrM,1510,1691,
T2P6,chrM:1865-2025,GCAAGGACTAACCCCTATACCTT,AAGGGGATTTAGAGGGTTCTGTG,59.82,60.27,23,23,47.83,47.83,chrM:1825-1847,chrM:2058-2081,256,43.36,chrM:1825-2081,1.1342,chrM,1847,2058,
T2P7,chrM:2234-2394,GTTCAAGCTCAACACCCACTAC,CCTTTCCTTATGAGCATGCCTG,59.96,59.90,22,22,50.00,50.00,chrM:2202-2223,chrM:2432-2454,252,39.68,chrM:2202-2454,1.3124,chrM,2223,2432,
T2P8,chrM:2591-2751,ATGTTTAACGGCCGCGGTA,TTGGTAGTTTAGGACCTGTGGG,60.58,59.89,19,22,52.63,50.00,chrM:2557-2575,chrM:2769-2791,234,45.30,chrM:2557-2791,2.1617,chrM,2575,2769,
T2P9,chrM:2918-3078,GCGAACTACTATACTCAATTGATCCA,CCTTTCGTACAGGGAGGAATTTG,59.39,59.83,26,23,38.46,47.83,chrM:2875-2900,chrM:3109-3132,257,43.97,chrM:2875-3132,3.2825,chrM,2900,3109,
T2P10,chrM:3290-3450,CAGGGTTTgttaagatggcagag,TGGTGAAGAGTTTTATGGCGTC,60.08,59.44,23,22,47.83,45.45,chrM:3221-3243,chrM:3456-3478,257,45.14,chrM:3221-3478,2.2123,chrM,3243,3456,
T2P11,chrM:3608-3768,ATACCCAACCCCCTGGTCA,TGGAGAGGTTAAAGGAGCCA,60.37,58.48,19,20,57.89,50.00,chrM:3576-3594,chrM:3776-3796,220,47.73,chrM:3576-3796,3.3568,chrM,3594,3776,
T2P12,chrM:3951-4111,CTAGTCTCAGGCTTCAACATCG,TAGGAGGTGTATGAGTTGGTCG,59.06,59.50,22,22,50.00,50.00,chrM:3924-3945,chrM:4151-4173,249,45.38,chrM:3924-4173,1.2556,chrM,3945,4151,
T2P13,chrM:4321-4481,CCCCTCAAACCTAAGAAATATGTCT,GATGGTAGAGTAGATGACGGGT,58.87,59.04,25,22,40.00,50.00,chrM:4249-4273,chrM:4486-4508,259,43.24,chrM:4249-4508,3.3063,chrM,4273,4486,
T2P14,chrM:4631-4791,GGCCTAGAAATAAACATGCTAGCT,TCTGGGACTCAGAAGTGAAAGG,59.46,59.89,24,22,41.67,50.00,chrM:4562-4585,chrM:4797-4819,257,38.13,chrM:4562-4819,3.7128,chrM,4585,4797,
T2P15,chrM:4942-5102,AACGTAAGCCTTCTCCTCACTC,AGGTGCGAGATAGTAGTAGGGT,60.29,60.09,22,22,50.00,50.00,chrM:4916-4937,chrM:5147-5169,253,40.32,chrM:4916-5169,1.2294,chrM,4937,5147,
T2P16,chrM:5315-5475,ATAGCCTCATCATCCCCACCAT,GAAGGCTCTTGGTCTGTATTTAACC,61.31,60.18,22,25,50.00,44.00,chrM:5289-5310,chrM:5520-5545,256,42.58,chrM:5289-5545,2.6221,chrM,5310,5520,
T2P17,chrM:5685-5845,CCCTTACTAGACCAATGGGACT,AGGTAAAATGGCTGAGTGAAGC,59.15,59.43,22,22,50.00,45.45,chrM:5650-5671,chrM:5870-5892,242,46.28,chrM:5650-5892,2.1426,chrM,5671,5870,
T2P18,chrM:6023-6183,CAGCTCTAAGCCTCCTTATTCGA,AGAGAGGGAGGTAAGAGTCAGAA,60.21,59.95,23,23,47.83,47.83,chrM:5994-6016,chrM:6206-6229,235,48.09,chrM:5994-6229,2.6389,chrM,6016,6206,
T2P19,chrM:6356-6516,CGTAGACCTAACCATCTTCTCCT,TGAGGTTGCGGTCTGTTAGTAG,59.32,60.29,23,22,47.83,50.00,chrM:6328-6350,chrM:6527-6549,221,47.06,chrM:6328-6549,1.9051,chrM,6350,6527,
T2P20,chrM:6697-6857,CTTATCCTACCAGGCTTCGGAA,AGTGTGGCGAGTCAGCTAAAT,59.83,60.24,22,21,50.00,47.62,chrM:6638-6659,chrM:6862-6883,245,42.86,chrM:6638-6883,0.8832,chrM,6659,6862,
T2P21,chrM:7011-7171,GCCTGACTGGCATTGTATTAGC,GGATAGGCCGAGAAAGTGTTGT,60.22,60.88,22,22,50.00,50.00,chrM:6957-6978,chrM:7183-7205,248,44.35,chrM:6957-7205,1.2366,chrM,6978,7183,
T2P22,chrM:7352-7482,GATTTGAGAAGCCTTCGCTTCG,CCTTTTTgaaaaagtcatggaggc,60.73,59.77,22,24,50.00,41.67,chrM:7315-7336,chrM:7497-7521,206,45.63,chrM:7315-7521,2.1340,chrM,7336,7497,
T2P23,chrM:7670-7830,AGGTCTACAAGACGCTACTTCC,TCGTTGACCTCGTCTGTTATGT,59.77,59.96,22,22,50.00,45.45,chrM:7605-7626,chrM:7838-7860,255,45.10,chrM:7605-7860,1.3774,chrM,7626,7838,
T2P24,chrM:7989-8149,TATTCCTAGAACCAGGCGACCT,CAGATTTCAGAGCATTGACCGT,60.90,59.51,22,22,50.00,45.45,chrM:7961-7982,chrM:8162-8184,223,47.53,chrM:7961-8184,2.5776,chrM,7982,8162,
T2P25,chrM:8329-8489,GCCCACTGTAAAGCTAACTTAGC,CGTTCATTTTGGTTCTCAGGGTT,60.15,60.21,23,23,47.83,43.48,chrM:8291-8313,chrM:8510-8533,242,37.60,chrM:8291-8533,3.1951,chrM,8313,8510,
T2P26,chrM:8690-8850,CCCACCTCCAAATATCTCATCAAC,CGAAAGCCTATAATCACTGTGCC,59.71,60.27,24,23,45.83,47.83,chrM:8619-8642,chrM:8856-8879,260,42.31,chrM:8619-8879,1.6095,chrM,8642,8856,
T2P27,chrM:9005-9165,CCATCAGCCTACTCATTCAACC,TGCAGGTAGAGGCTTACTAGAAG,59.31,59.57,22,23,50.00,47.83,chrM:8962-8983,chrM:9171-9194,232,46.12,chrM:8962-9194,1.8664,chrM,8983,9171,
T2P28,chrM:9363-9523,CTTCCACTCCATAACGCTCCT,AGTGCCCTCCTAATTGGGG,60.04,59.19,21,19,52.38,57.89,chrM:9313-9333,chrM:9533-9552,239,47.28,chrM:9313-9552,3.9539,chrM,9333,9533,
T2P29,chrM:9712-9872,CCGAAACCAAATAATTCAAGCACTG,TCGAAGCCAAAGTGATGTTTGG,60.18,60.22,25,22,40.00,45.45,chrM:9670-9694,chrM:9891-9913,243,43.21,chrM:9670-9913,3.0037,chrM,9694,9891,
T2P30,chrM:10075-10235,CGTTAACTTCCAATTAACTAGTTTTGAC,GGAGGGCAATTTCTAGATCAAATAATAA,58.44,58.55,28,28,32.14,32.14,chrM:10012-10039,chrM:10244-10272,260,37.69,chrM:10012-10272,18.7437,chrM,10039,10244,
T2P31,chrM:10408-10568,GGCCTATGAGTGACTACAAAAAGG,GTGTTGAGGGTTATGAGAGTAGCT,59.89,60.38,24,24,45.83,45.83,chrM:10363-10386,chrM:10597-10621,258,34.50,chrM:10363-10621,4.5000,chrM,10386,10597,
T2P32,chrM:10747-10907,ACACATATGGCCTAGACTACGTAC,GTCAGGTAGTTAGTATTAGGAGGGG,60.01,59.77,24,25,45.83,48.00,chrM:10716-10739,chrM:10948-10973,257,39.30,chrM:10716-10973,2.6661,chrM,10739,10948,
T2P33,chrM:11126-11286,CTCCTTAATTATAACATTCACAGCCACA,AGGAGTTTGATAGTTCTTGGGCAG,60.51,61.11,28,24,35.71,45.83,chrM:11070-11097,chrM:11303-11327,257,44.75,chrM:11070-11327,5.4805,chrM,11097,11303,
T2P34,chrM:11480-11640,CAATAGTACTTGCCGCAGTACTC,GGATGAGAATGGCTGTTACTACG,59.72,59.46,23,23,47.83,47.83,chrM:11441-11463,chrM:11646-11669,228,44.74,chrM:11441-11669,1.3843,chrM,11463,11646,
T2P35,chrM:11824-11984,ACTACGAACGCACTCACAGT,AGTGAGCCCCATTGTGTTGT,59.84,60.34,20,20,50.00,50.00,chrM:11756-11775,chrM:11995-12015,259,45.56,chrM:11756-12015,1.6912,chrM,11775,11995,
T2P36,chrM:12159-12319,CATCATTACCGGGTTTTCCTCTTG,GTCAGGGTTAGGGTGGTTATAGTAG,60.43,60.24,24,25,45.83,48.00,chrM:12114-12137,chrM:12349-12374,260,40.00,chrM:12114-12374,2.4930,chrM,12137,12349,
T2P37,chrM:12526-12686,TTCATGTGCCTAGACCAAGAAG,GTTGGAATAGGTTGTTAGCGGT,58.57,59.17,22,22,45.45,45.45,chrM:12495-12516,chrM:12732-12754,259,38.22,chrM:12495-12754,4.3486,chrM,12516,12732,
T2P38,chrM:12877-13037,AGCAGCCATTCAAGCAATCC,TGAGTGGAGTAGGGCTGAGA,59.68,59.82,20,20,50.00,55.00,chrM:12833-12852,chrM:13063-13083,250,52.40,chrM:12833-13083,4.3860,chrM,12852,13063,
T2P39,chrM:13232-13392,TGCGCCCTTACACAAAATGAC,ATGGTGAGGGAGGTTGAAGTG,60.24,60.17,21,21,47.62,52.38,chrM:13206-13226,chrM:13439-13460,254,42.91,chrM:13206-13460,1.1442,chrM,13226,13439,
T2P40,chrM:13585-13745,GCCTGAGCCCTATCTATTACTCT,GTAGAGGGGGATTGTTGTTTGG,59.18,59.43,23,22,47.83,50.00,chrM:13551-13573,chrM:13769-13791,240,49.17,chrM:13551-13791,2.9670,chrM,13573,13769,
T2P41,chrM:13930-14090,TCTCCAACATACTCGGATTCTACC,TGAGTGGGAAGAAGAAAGAGAGG,59.95,59.95,24,23,45.83,47.83,chrM:13900-13923,chrM:14099-14122,222,44.59,chrM:13900-14122,1.6117,chrM,13923,14099,
T2P42,chrM:14244-14404,AGTAACTACTACTAATCAACGCCCAT,CGATGGCTATTGAGGAGTATCCTG,60.22,60.91,26,24,38.46,50.00,chrM:14205-14230,chrM:14435-14459,254,48.03,chrM:14205-14459,4.5388,chrM,14230,14435,
T2P43,chrM:14594-14754,GAATAATAACACACCCGACCACAC,GAGTGGTTAATTAATTTTATTAGGGGGT,60.43,58.43,24,28,45.83,32.14,chrM:14543-14566,chrM:14768-14796,253,39.53,chrM:14543-14796,6.2339,chrM,14566,14768,
T2P44,chrM:14923-15083,ACAGGACTATTCCTAGCCATGC,AGGACATAGCCTATGAAGGCTG,60.16,59.89,22,22,50.00,50.00,chrM:14884-14905,chrM:15122-15144,260,47.69,chrM:14884-15144,1.9176,chrM,14905,15122,
T2P45,chrM:15292-15452,CACCCTCACACGATTCTTTACC,GGTATAATTGTCTGGGTCGCCT,59.51,60.42,22,22,50.00,50.00,chrM:15264-15285,chrM:15495-15517,253,46.64,chrM:15264-15517,1.7857,chrM,15285,15495,
T2P46,chrM:15644-15804,GATCCGTCCCTAACAAACTAGGA,AGGATTGTTGTGAAGTATAGTACGG,59.83,58.96,23,25,47.83,40.00,chrM:15590-15612,chrM:15809-15834,244,44.26,chrM:15590-15834,2.7357,chrM,15612,15809,
T2P47,chrM:15961-16121,CACCAGTCTTGTAAACCGGAGA,AGGTGGTCAAGTATTTATGGTACCG,60.49,60.94,22,25,50.00,44.00,chrM:15909-15930,chrM:16127-16152,243,40.33,chrM:15909-16152,3.4681,chrM,15930,16127,
T2P48,chrM:16272-16432,cacacatcaactgcaactCCAA,TCACTTTAGCTACCCCCAAGTG,60.16,60.23,22,22,45.45,50.00,chrM:16231-16252,chrM:16464-16486,255,50.59,chrM:16231-16486,3.4954,chrM,16252,16464,

Designed by MP-Ref